Abstract The volumetric mass transfer coefficient, usually denoted by kLa, is an important parameter for both the design, scale-up and monitoring of aerated bioreactors. Significant research efforts have been expended over the last decades in order to develop reliable and easy to apply methodologies for determining kLa using aeration experiment and fast oxygen (polarographic) probes. These methodologies have been reviewed in this study followed by the presentation of a new and promising methodology for kLa determination, which can be used when data from step experiments are not available. Step and aeration experiments have been carried out in laboratory scale bioreactors and the experimental data were used for the validation of the proposed methodology and also to critically assess all alternative methodologies for estimating kLa.

中文翻译：

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体积传质系数 (kLa) 的估计 - 经典方法的回顾和新方法的贡献

摘要 体积传质系数通常用 kLa 表示，是曝气生物反应器设计、放大和监测的重要参数。在过去的几十年中，为了开发可靠且易于应用的方法来使用曝气实验和快速氧气（极谱）探针确定 kLa，已经进行了大量的研究工作。本研究对这些方法进行了审查，然后介绍了一种新的、有前途的 kLa 测定方法，可在无法获得分步实验数据时使用。步骤和曝气实验已在实验室规模的生物反应器中进行，实验数据用于验证所提出的方法，并严格评估所有用于估计 kLa 的替代方法。