Abstract In modern aquaculture, simple and rapid methods of predicting fish ovulation are needed to produce seedlings in a stable and efficient manner. Ovulation time is variable due to environmental and physiological factors, while 17α,20β-dihydroxy-4-pregnen-3-one (DHP), an indicator of ovulation, increases its concentration rapidly just before spawning. In this paper, we developed a biosensor system in which immune and enzymatic reaction assays were combined to monitor DHP levels with low environmental loads. We attempted to quantify DHP by analyzing the decrease rate in the output current after the immunoreaction using the amperometric method as a transfer inhibition degree of the enzyme-generated electrons generated by the formation of the antigen-antibody complex. DHP standard samples were measured under investigated optimal conditions and a specific response with a good linear correlation was obtained between the current decrease rate of the sensor system and the DHP concentration in a range of 7.81∼500 pg ml−1. Further, DHP levels in goldfish (Carassius auratus) during the ovulation period obtained using the sensor system were compared with those of using the conventional method (ELISA) and a good correlation was obtained. These findings suggest that the proposed sensor system can detect DHP quickly and easily compared with the conventional method.

中文翻译：

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一种用于测量鱼类成熟诱导激素的酶功能化免疫传感器的研制

摘要 在现代水产养殖中，需要一种简单、快速的预测鱼类排卵的方法，以稳定高效地生产幼苗。排卵时间因环境和生理因素而异，而 17α,20β-dihydroxy-4-pregnen-3-one (DHP)（排卵指标）会在产卵前迅速增加其浓度。在本文中，我们开发了一种生物传感器系统，其中免疫和酶促反应测定相结合，以监测低环境负荷下的 DHP 水平。我们试图通过使用安培法分析免疫反应后输出电流的下降率来量化 DHP，作为抗原-抗体复合物形成所产生的酶生成电子的转移抑制程度。在研究的最佳条件下测量 DHP 标准样品，在 7.81∼500 pg ml-1 范围内，传感器系统的电流下降率与 DHP 浓度之间获得了具有良好线性相关性的特定响应。此外，将使用传感器系统获得的排卵期金鱼 (Carassius auratus) 的 DHP 水平与使用常规方法 (ELISA) 获得的 DHP 水平进行比较，并获得了良好的相关性。这些发现表明，与传统方法相比，所提出的传感器系统可以快速、轻松地检测 DHP。将使用传感器系统获得的排卵期金鱼 (Carassius auratus) 的 DHP 水平与使用常规方法 (ELISA) 的水平进行比较，并获得了良好的相关性。这些发现表明，与传统方法相比，所提出的传感器系统可以快速、轻松地检测 DHP。将使用传感器系统获得的排卵期金鱼 (Carassius auratus) 的 DHP 水平与使用常规方法 (ELISA) 的水平进行比较，并获得了良好的相关性。这些发现表明，与传统方法相比，所提出的传感器系统可以快速、轻松地检测 DHP。