当前位置: X-MOL 学术Acta Histochem. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Biochemical, immunohistochemical and morphometrical investigation of the effect of thymoquinone on the rat testis following exposure to a 900-MHz electromagnetic field.
Acta Histochemica ( IF 2.3 ) Pub Date : 2019-11-26 , DOI: 10.1016/j.acthis.2019.151467
Ahmad Yahyazadeh 1 , Berrin Zuhal Altunkaynak 2 , Suleyman Kaplan 3
Affiliation  

Long-term use of cell phones emitting electromagnetic fields (EMFs) have raised concerns regarding public health in recent year. We aimed to investigate the possible effects of 900 MHz EMF exposure (60 min/day for 28 days) on the rat testis. Another objective was to determine whether the deleterious effect of EMF radiation would be reduced by the administration of thymoquinone (TQ) (10 mg/kg/day). Twenty-four male adult Wistar albino rats were randomly selected, then assigned into four groups as followControl, EMF, TQ and EMF + TQ. Testicular samples were analyzed using histological, stereological, biochemical and immunohistochemical techniques. Total numbers of primary spermatocytes and spermatids as well as Leydig cells were significantly decreased in the EMF group compared to the Control group (p < 0.05). In the EMF + TQ group, the total number of primary spermatocytes was significantly increased compared to the EMF group (p < 0.05). Superoxide dismutase (SOD) activity was significantly increased in the EMF group compared to the Control group (p < 0.05). Also, serum testosterone levels and wet weight of testes were significantly decreased in the EMF group compared to the Control group (p < 0.05). Our findings suggested that exposure to a 900 MHz EMF had adverse effects on rat testicular tissue and that the administration of TQ partially mitigated testicular oxidative damages caused by EMF radiation.

中文翻译:

暴露于900 MHz电磁场后,胸腺醌对大鼠睾丸的影响的生化,免疫组化和形态计量学研究。

近年来,长期使用发射电磁场(EMF)的手机引起了人们对公共健康的关注。我们旨在研究900 MHz EMF暴露(每天60分钟/每天,共28天)对大鼠睾丸的可能影响。另一个目的是确定施用胸腺醌(TQ)(10 mg / kg /天)是否会降低EMF辐射的有害作用。随机选择24只成年Wistar白化病雄性大鼠,然后将其分为4组,分别为Control,EMF,TQ和EMF + TQ。使用组织学,立体学,生化和免疫组化技术分析睾丸样品。与对照组相比,EMF组的原代精母细胞和精子细胞总数以及Leydig细胞总数明显减少(p <0.05)。在EMF + TQ组中,与EMF组相比,原代精子细胞的总数显着增加(p <0.05)。与对照组相比,EMF组中的超氧化物歧化酶(SOD)活性显着提高(p <0.05)。而且,与对照组相比,EMF组的血清睾丸激素水平和睾丸湿重显着降低(p <0.05)。我们的发现表明,暴露于900 MHz EMF对大鼠睾丸组织有不利影响,而TQ的使用可部分缓解EMF辐射引起的睾丸氧化损伤。与对照组相比,EMF组的血清睾丸激素水平和睾丸湿重显着降低(p <0.05)。我们的发现表明,暴露于900 MHz EMF对大鼠睾丸组织有不利影响,而TQ的使用可部分缓解由EMF辐射引起的睾丸氧化损伤。与对照组相比,EMF组的血清睾丸激素水平和睾丸湿重显着降低(p <0.05)。我们的发现表明,暴露于900 MHz EMF对大鼠睾丸组织有不利影响,而TQ的使用可部分缓解EMF辐射引起的睾丸氧化损伤。
更新日期:2019-11-27
down
wechat
bug