The main objective of this study was to investigate the effect of low-level light emitting diode (LED) irradiation on the metabolite profile of pak-choi. A total of 633 different molecular features (MFs) were identified among sample groups (initial, dark-treated, light-treated) using an untargeted metabolomic approach. The identified metabolites were associated with 24 different metabolic pathways. Four of the pathways including carbon pool by folate, folate biosynthesis, thiamine metabolism, and glutathione metabolism, all of which are associated with vitamin biosynthesis, changed significantly. Metabolites in four of the pathways exhibited significant differences from the control in response to LED irradiation. Additionally, porphyrin and chlorophyll metabolism, as well as glucosinolate biosynthesis, riboflavin metabolism, and carotenoid biosynthesis were positively induced by LED irradiation. These results indicate that postharvest LED illumination represents a potential tool for modifying the metabolic profile of pak-choi to maintain quality and nutritional levels.

中文翻译：

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一种无针对性的代谢组学方法显示了白菜（甘蓝型油菜（L.）Makino var。communis Tsen等，Lee）对LED辐射的响应，表明维生素的代谢在采后发生了显着变化。

这项研究的主要目的是研究低水平发光二极管（LED）辐射对小白菜代谢物谱的影响。使用非靶向代谢组学方法，在样品组（初始，暗处理，亮处理）中鉴定出总共633种不同的分子特征（MF）。鉴定出的代谢产物与24种不同的代谢途径相关。叶酸的碳库，叶酸的生物合成，硫胺素代谢和谷胱甘肽代谢中的四个途径，都与维生素的生物合成有关，发生了显着变化。四个途径中的代谢物在响应LED照射方面显示出与对照相比的显着差异。此外，卟啉和叶绿素代谢以及芥子油苷的生物合成，核黄素代谢，LED照射能积极诱导类胡萝卜素和类胡萝卜素的生物合成。这些结果表明，收获后的LED照明代表了一种潜在的工具，可用于改变小白菜的代谢特性以维持质量和营养水平。