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Codon Selection Affects Recruitment of Ribosome-Associating Factors during Translation.
ACS Synthetic Biology ( IF 3.7 ) Pub Date : 2019-12-10 , DOI: 10.1021/acssynbio.9b00344
Alejandra M Rojano-Nisimura 1 , Katie Haning 2 , Justin Janovsky 1 , Kevin A Vasquez 2 , Jeffrey P Thompson 2 , Lydia M Contreras 2
Affiliation  

An intriguing aspect of protein synthesis is how cotranslational events are managed inside the cell. In this study, we developed an in vivo bimolecular fluorescence complementation assay coupled to SecM stalling (BiFC-SecM) to study how codon usage influences the interactions of ribosome-associating factors that occur cotranslationally. We profiled ribosomal associations of a number of proteins, and observed differential association of chaperone proteins TF, DnaK, GroEL, and translocation factor Ffh as a result of introducing synonymous codon substitutions that change the affinity of the translating sequence to the ribosomal anti-Shine-Dalgarno (aSD) sequence. The use of pausing sequences within proteins regulates their transit within the translating ribosome. Our results indicate that the dynamics between cellular factors and the new polypeptide chain are affected by how codon composition is designed. Furthermore, associating factors may play a role in processes including protein quality control (folding and degradation) and cellular respiration.

中文翻译:

密码子选择影响翻译过程中核糖体相关因子的募集。

蛋白质合成的一个有趣方面是如何在细胞内部管理共翻译事件。在这项研究中,我们开发了一种与SecM失速(BiFC-SecM)偶联的体内双分子荧光互补测定法,以研究密码子使用如何影响共翻译发生的核糖体相关因子之间的相互作用。我们介绍了许多蛋白质的核糖体缔合情况,并观察到了伴侣蛋白TF,DnaK,GroEL和易位因子Ffh的差异缔合,这是由于引入了同义密码子取代,从而改变了翻译序列对核糖体抗Shine- Dalgarno(aSD)序列。蛋白质中暂停序列的使用可调节其在翻译核糖体中的转运。我们的结果表明,细胞因子与新多肽链之间的动力学受到密码子组成设计方式的影响。此外,相关因素可能在包括蛋白质质量控​​制(折叠和降解)和细胞呼吸在内的过程中发挥作用。
更新日期:2019-12-11
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