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Native Chromatin Proteomics Reveals a Role for Specific Nucleoporins in Heterochromatin Organization and Maintenance.
Molecular Cell ( IF 14.5 ) Pub Date : 2019-11-26 , DOI: 10.1016/j.molcel.2019.10.018
Nahid Iglesias 1 , Joao A Paulo 2 , Antonis Tatarakis 1 , Xiaoyi Wang 1 , Amanda L Edwards 3 , Natarajan V Bhanu 4 , Benjamin A Garcia 4 , Wilhelm Haas 5 , Steven P Gygi 2 , Danesh Moazed 1
Affiliation  

Spatially and functionally distinct domains of heterochromatin and euchromatin play important roles in the maintenance of chromosome stability and regulation of gene expression, but a comprehensive knowledge of their composition is lacking. Here, we develop a strategy for the isolation of native Schizosaccharomyces pombe heterochromatin and euchromatin fragments and analyze their composition by using quantitative mass spectrometry. The shared and euchromatin-specific proteomes contain proteins involved in DNA and chromatin metabolism and in transcription, respectively. The heterochromatin-specific proteome includes all proteins with known roles in heterochromatin formation and, in addition, is enriched for subsets of nucleoporins and inner nuclear membrane (INM) proteins, which associate with different chromatin domains. While the INM proteins are required for the integrity of the nucleolus, containing ribosomal DNA repeats, the nucleoporins are required for aggregation of heterochromatic foci and epigenetic inheritance. The results provide a comprehensive picture of heterochromatin-associated proteins and suggest a role for specific nucleoporins in heterochromatin function.

中文翻译:

天然染色质蛋白质组学揭示了特定核蛋白在异染色质组织和维持中的作用。

异染色质和常染色质的空间和功能上不同的结构域在维持染色体稳定性和调节基因表达中起着重要作用,但缺乏对其组成的全面了解。在这里,我们制定了一种策略,用于分离天然粟酒裂殖酵母异染色质和常染色质片段,并通过定量质谱分析其组成。共有蛋白和常染色质特异的蛋白质组分别包含参与DNA和染色质代谢以及转录的蛋白质。异染色质特异性蛋白质组包括在异染色质形成中具有已知作用的所有蛋白质,此外,富含与不同染色质结构域相关的核孔蛋白和内核膜(INM)蛋白的子集。虽然INM蛋白是包含核糖体DNA重复序列的核仁完整性所必需的,但核孔蛋白却是异色病灶聚集和表观遗传所必需的。结果提供了异染色质相关蛋白的全面描述,并暗示了特定核孔蛋白在异染色质功能中的作用。
更新日期:2019-11-27
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