To determine the role of piscine anti-viral cytotoxic cells, we analyzed the response of channel catfish to Ictalurid herpesvirus 1, commonly designated channel catfish virus (CCV). Peripheral blood leukocytes (PBL) from catfish immunized with MHC-matched, CCV-infected G14D cells (G14D-CCV) showed marked lysis of G14D-CCV but little to no lysis of uninfected allogenic (3B11) or syngeneic (G14D) cells. Expansion of effectors by in vitro culture in the presence of irradiated G14D-CCV cells generated cultures with enhanced cytotoxicity and often broader target range. Cytotoxic effectors expressed rearranged TCR genes, perforin, granzyme, and IFN-γ. Four clonal cytotoxic lines were developed and unique TCR gene rearrangements including γδ were detected. Furthermore, catfish CTL clones were either CD4+/CD8- or CD4-/CD8-. Two CTL lines showed markedly enhanced killing of G14D-CCV targets, while the other two lines displayed a broader target range. Collectively, catfish virus-specific CTL display unique features that illustrate the diversity of the ectothermic vertebrate immune response.

中文翻译：

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通道cat鱼Ictalurus punctatus中抗病毒细胞毒性T淋巴细胞的克隆和鉴定。

为了确定鱼类抗病毒细胞毒性细胞的作用，我们分析了通道cat鱼对Ictalurid疱疹病毒1（通常称为通道cat鱼病毒（CCV））的反应。用MHC匹配的CCV感染的G14D细胞（G14D-CCV）免疫了cat鱼的外周血白细胞（PBL）显示出G14D-CCV的显着裂解，但未感染的同种异体（3B11）或同系（G14D）细胞几乎没有裂解。通过在有辐射的G14D-CCV细胞存在下进行体外培养来扩大效应子，从而产生具有增强的细胞毒性和通常更广泛的靶标范围的培养物。细胞毒性效应物表达了重排的TCR基因，穿孔素，颗粒酶和IFN-γ。开发了四个克隆细胞毒株，并检测了包括γδ在内的独特的TCR基因重排。此外，cat鱼CTL克隆为CD4 + / CD8-或CD4- / CD8-。两条CTL品系显示出对G14D-CCV目标的杀伤力明显增强，而其他两条品系显示出更广泛的靶标范围。总体上，cat鱼病毒特异的CTL显示出独特的功能，这些特征说明了外温脊椎动物免疫应答的多样性。