Purpose

This study was designed to evaluate the protective effects of allicin against diclofenac sodium induced hepatonephro toxicity in rats.

Methods

Sixty male Wister albino rats were assigned into six groups. The control group received calcium carbonate and corn starch. 2^nd group received diclofenac sodium (2 mg/kg bw orally) for 30 days. 3^rd group received allicin (45 mg/kg bw orally) for 30 days. 4^th group administrated diclofenac sodium as in the 2^nd group and allicin (15 mg/kg bw orally) for 30 days. 5^th group received diclofenac sodium as in the 2^nd group and allicin (30 mg/kg bw orally) for 30 days. 6^th group received diclofenac sodium as 2^nd and allicin (45 mg/kg bw orally) for 30 days.

Results

Diclofenac sodium significantly elevated activities of serum aspartate aminotransferase and alanine aminotransferase and serum levels of creatinine and urea. In addition, it induced hyperglycemia, lipid peroxidation, pathological alteration and caspase 3 protein expression in hepatic and renal tissues. However, it decreased reduced glutathione concentration and proliferating cell nuclear antigen protein expression in hepatic tissues. In contrast, allicin modulated the diclofenac sodium induced alteration in liver and kidney functions and structures dose dependently.

Conclusion

This study indicated that allicin had potential preventive effects against diclofenac sodium induced hepatonephro toxicity in rats.

中文翻译：

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大蒜素可通过降低氧化应激和caspase 3蛋白表达来调节双氯芬酸钠引起的大鼠肝酮中毒。

目的

本研究旨在评估大蒜素对双氯芬酸钠诱导的大鼠肝肾毒性的保护作用。

方法

将六十只雄性Wister白化病大鼠分为六组。对照组接受碳酸钙和玉米淀粉。2^第二组接收到的双氯芬酸钠（2毫克/千克体重口服）30天。3 ^RD组接受大蒜素（45毫克/公斤体重口服）30天。4^个组施用双氯芬酸钠如在2^次组和大蒜素（15毫克/公斤体重口服）30天。5^个接收的组双氯芬酸钠如在2^次组和大蒜素（30毫克/公斤体重口服）30天。6^个组接受双氯芬酸钠作为2^次和大蒜素（45毫克/公斤体重口服）30天。

结果

双氯芬酸钠显着提高了血清天冬氨酸转氨酶和丙氨酸转氨酶的活性以及血清肌酐和尿素的水平。此外，它诱导肝和肾组织中的高血糖症，脂质过氧化，病理改变和caspase 3蛋白表达。但是，它减少了肝组织中降低的谷胱甘肽浓度和增殖细胞核抗原蛋白的表达。相反，大蒜素调节双氯芬酸钠诱导的肝，肾功能和结构改变，其剂量依赖性。

结论

这项研究表明，大蒜素对双氯芬酸钠诱导的大鼠肝肾毒性具有潜在的预防作用。