Hypertonic saccharide solution delays pyroptosis in murine macrophages regardless of the membrane binding of gasdermin D N-terminal.,European Journal of Immunology

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Hypertonic saccharide solution delays pyroptosis in murine macrophages regardless of the membrane binding of gasdermin D N-terminal.
European Journal of Immunology ( IF 4.5 ) Pub Date : 2019-12-10 , DOI: 10.1002/eji.201948230
Jiashuo Zheng _{1,

2} , Dingyu Wang ₃ , Qianyue Chen ₃ , Qiyao Liu ₃ , Zhaoyu Lin ₃ , Qiongyuan Hu _{1,

2} , Xiuwen Wu _{1,

2} , Xiang Gao ₃ , Qiurong Li ₁ , Jianan Ren _{1,

2}

Affiliation

Pyroptosis is a type of programmed lytic cell death that could be activated by either the canonical or noncanonical inflammasome pathway. In this study, we aimed to examine the effect of hypertonic solution on noncanonical pyroptosis in macrophage. We found that although hypertonic solution had a general inhibitory effect on noncanonical pyroptosis, the underlying mechanism varied by the solute causing hypertonicity. Specifically, hypertonic NaCl or KCl solution inhibited the cleavage of gasdermin D, the pore-forming protein in pyroptosis, whereas hypertonic saccharide solution did not affect the cleavage or membrane binding of gasdermin D. In this case, nevertheless, pyroptosis was still inhibited as evidenced by the preserved mitochondria activity and cell membrane permeability.

中文翻译：

高渗糖溶液可延缓鼠巨噬细胞的焦磷酸化作用，而与Gasdermin D N末端的膜结合无关。

细胞凋亡是一种程序性裂解细胞死亡，可以通过规范或非规范性炎症小体途径激活。在这项研究中，我们旨在检查高渗溶液对巨噬细胞非规范性凋亡的影响。我们发现，尽管高渗溶液对非规范性焦细胞凋亡具有一般的抑制作用，但其潜在机制因引起高渗的溶质而异。具体而言，高渗性NaCl或KCl溶液抑制了胃泌素D的裂解，即成孔蛋白gasdermin D的裂解，而高渗糖溶液不影响Gasdermin D的裂解或膜结合。在这种情况下，如所证明的，裂解仍被抑制通过保留的线粒体活性和细胞膜通透性。

更新日期：2019-12-11

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