Potato leafroll virus (PLRV), genus Polerovirus, family Luteoviridae, is a major pathogen of potato worldwide. PLRV is transmitted among host plants by aphids in a circulative-nonpropagative manner. Previous studies have demonstrated that PLRV infection increases aphid fecundity on, and attraction to, infected plants as compared to controls. However, the molecular mechanisms mediating this relationship are still poorly understood. In this study, we measured the impact of PLRV infection on plant-aphid interactions and plant chemistry in two hosts: Solanum tuberosum and Nicotiana benthamiana. Our study demonstrates that PLRV infection attenuates the induction of aphid-induced jasmonic acid and ethylene in S. tuberosum and N. benthamiana. Using transient expression experiments, insect bioassays and chemical analysis, we show that expression of three PLRV proteins (P0, P1, and P7) mediate changes in plant-aphid interactions and inhibition of aphid-induced jasmonic acid and ethylene in N. benthamiana. This study enhances our understanding of the plant-vector-pathogen interface by elucidating new mechanisms by which plant viruses transmitted in a circulative manner can manipulate plant hosts.

中文翻译：

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马铃薯卷叶病毒是一种马铃薯卷叶病毒，它利用三种病毒蛋白改变植物与载体的相互作用。


马铃薯卷叶病毒（PLRV）属于黄病毒科马铃薯卷叶病毒属，是全世界马铃薯的主要病原体。 PLRV 通过蚜虫以循环非繁殖方式在寄主植物之间传播。先前的研究表明，与对照相比，PLRV 感染增加了受感染植物上的蚜虫繁殖力和吸引力。然而，介导这种关系的分子机制仍然知之甚少。在这项研究中，我们测量了 PLRV 感染对两种宿主（马铃薯和烟草）中植物-蚜虫相互作用和植物化学的影响。我们的研究表明，PLRV 感染减弱了马铃薯和本塞姆氏烟草中蚜虫诱导的茉莉酸和乙烯的诱导。通过瞬时表达实验、昆虫生物测定和化学分析，我们发现三种 PLRV 蛋白（P0、P1 和 P7）的表达介导植物与蚜虫相互作用的变化以及对本塞姆氏烟草中蚜虫诱导的茉莉酸和乙烯的抑制。这项研究通过阐明以循环方式传播的植物病毒可以操纵植物宿主的新机制，增强了我们对植物-载体-病原体界面的理解。