Arabidopsis eceriferum (cer) mutants with unique alterations in their rosette leaf cuticular wax accumulation and composition established by gas chromatography have been investigated using attenuated total reflection (ATR)-Fourier transform infrared (FTIR) spectroscopy in combination with univariate and multivariate analysis. Objectives of this study were to evaluate the utility of ATR-FTIR for detection of chemical diversity in leaf cuticles, obtain spectral profiles of cer mutants in comparison with the wild type, and identify changes in leaf cuticles caused by drought stress. FTIR spectra revealed both genotype- and treatment-dependent differences in the chemical make-up of Arabidopsis leaf cuticles. Drought stress caused specific changes in the integrated area of the CH3 peak, asymmetrical and symmetrical CH2 peaks, ester carbonyl peak and the peak area ratio of ester CO to CH2 asymmetrical vibration. CH3 peak positively correlated with the total wax accumulation. Thus, ATR-FTIR spectroscopy is a valuable tool that can advance our understanding of the role of cuticle chemistry in plant response to drought and allow selection of superior drought-tolerant varieties from large genetic resources.

中文翻译：

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中红外光谱法是一种通过改变叶表皮蜡质来选择拟南芥基因型的快速筛选方法。

已使用衰减全反射（ATR）-傅里叶变换红外（FTIR）光谱结合单变量和多变量分析研究了玫瑰花叶表皮蜡积累和组成由气相色谱法确定的具有独特改变的拟南芥（cer）突变体。这项研究的目的是评估ATR-FTIR在检测叶片表皮化学多样性方面的实用性，获得与野生型相比cer突变体的光谱图，并鉴定干旱胁迫引起的叶片表皮变化。FTIR光谱揭示了拟南芥叶角质层化学组成的基因型和治疗依赖性差异。干旱胁迫导致CH3峰积分区域，CH2峰不对称和对称出现特定变化，酯羰基峰和酯CO与CH2的不对称振动的峰面积比。CH3峰与总蜡积累呈正相关。因此，ATR-FTIR光谱学是一种有价值的工具，可以增进我们对表皮化学在植物对干旱的反应中的作用的了解，并允许从大量的遗传资源中选择优良的耐旱品种。