当前位置: X-MOL 学术ACS Infect. Dis. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Trichomonas vaginalis Motility Is Blocked by Drug-Free Thermosensitive Hydrogel.
ACS Infectious Diseases ( IF 4.0 ) Pub Date : 2019-11-25 , DOI: 10.1021/acsinfecdis.9b00243
Sophia Malli 1, 2 , Philippe M Loiseau 3 , Kawthar Bouchemal 2
Affiliation  

Trichomonas vaginalis motility in biological fluids plays a prominent, but understudied, role in parasite infectivity. In this study, the ability of a thermosensitive hydrogel (pluronic F127) to physically immobilize T. vaginalis was investigated. Blocking parasite motility could prevent its attachment to the mucosa, thus reducing the acquisition of the infection. The trajectory of individual parasites was monitored by multiple particle tracking. Mean square displacement, diffusivity, and velocity were calculated from x, y coordinates during time. Major results are that T. vaginalis exhibited different types of trajectories in a diluted solution composed of lactate buffer similar to "run-and-tumble" motion reported for flagellated bacteria. The fastest T. vaginalis specimen moves with a velocity of 19 μm/s. Observation of T. vaginalis movements showed that the cell body remains rigid during swimming and that the propulsive forces necessary to generate the movement are the result of flagellar beating. Parasite motility was partially slowed down using hydroxyethylcellulose hydrogel, used as a reference for the development of vaginal microbicides, while 100% of T. vaginalis were immobile in F127 hydrogel. Once completed by biological investigations on mice, this report suggests using drug-free formulation composed of F127 as a new strategy to prevent T. vaginalis attachment to the mucosa. The concept will be extended to other flagellated organisms where the motility is driven by cilia and flagella.

中文翻译:

阴道毛滴虫的运动被无药热敏性水凝胶阻止。

阴道毛滴虫在生物体液中的运动在寄生虫感染性中起着重要但未被充分研究的作用。在这项研究中,研究了热敏性水凝胶(普流尼克F127)物理固定阴道锥虫的能力。阻止寄生虫运动可以阻止其附着在粘膜上,从而减少感染的获得。单个寄生虫的轨迹通过多粒子跟踪进行监控。根据时间上的x,y坐标计算均方位移,扩散率和速度。主要结果是阴道锥虫在由乳酸缓冲液组成的稀释溶液中表现出不同类型的轨迹,这类似于有鞭毛细菌的“奔跑”运动。最快的阴道T.标本移动速度为19μm/ s。观察T。阴道运动表明,游泳时细胞体保持僵硬,而产生运动所需的推进力是鞭毛殴打的结果。使用羟乙基纤维素水凝胶可部分降低寄生虫的运动能力,羟乙基纤维素水凝胶可作为阴道杀菌剂发展的参考,而100%的阴道锥虫不能在F127水凝胶中固定。一旦通过对小鼠的生物学研究完成,该报告建议使用由F127组成的无药制剂作为防止阴道锥虫附着在粘膜上的新策略。该概念将扩展到由纤毛和鞭毛驱动运动的其他鞭毛生物。使用羟乙基纤维素水凝胶可部分降低寄生虫的运动能力,羟乙基纤维素水凝胶可作为阴道杀菌剂发展的参考,而100%的阴道锥虫不能在F127水凝胶中固定。一旦通过对小鼠的生物学研究完成,该报告建议使用由F127组成的无药制剂作为预防阴道锥虫附着在粘膜上的新策略。该概念将扩展到由纤毛和鞭毛驱动运动的其他鞭毛生物。使用羟乙基纤维素水凝胶可部分降低寄生虫的运动能力,羟乙基纤维素水凝胶可作为阴道杀菌剂发展的参考,而100%的阴道锥虫不能在F127水凝胶中固定。一旦通过对小鼠的生物学研究完成,该报告建议使用由F127组成的无药制剂作为预防阴道锥虫附着在粘膜上的新策略。该概念将扩展到由纤毛和鞭毛驱动运动的其他鞭毛生物。阴道粘膜附着。该概念将扩展到由纤毛和鞭毛驱动运动的其他鞭毛生物。阴道粘膜附着。该概念将扩展到由纤毛和鞭毛驱动运动的其他鞭毛生物。
更新日期:2019-11-28
down
wechat
bug