Abstract Butyl acetate is a versatile chemical due to its excellent solvency for polymers, resins, and oils. In this work, the butyl acetate synthesis was studied by the esterification of n-butanol with acetic acid using the commercial immobilized lipase Novozym® 435. The acetic acid and n-butanol concentrations have to be limited in the enzymatic synthesis of this ester due to the deleterious effects caused by these reagents on the enzyme. Three methods of acetic acid addition (single, stepwise and continuous addition) were investigated to minimize the acid concentration. A significant drop on acid conversion was observed in the presence of a higher concentration of the reagents when single or stepwise acid addition was used. However, a solvent-free system using a continuous addition of acetic acid favored butyl acetate synthesis, reaching a conversion of 84% at 60 °C, employing 5 wt% of enzyme and n-butanol/acetic acid final molar ratio equal to 1/1.

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乙酸加成方法对乙酸丁酯酶法合成的影响

摘要 乙酸丁酯是一种多功能化学品，因为它对聚合物、树脂和油类具有出色的溶解能力。在这项工作中，使用商业固定化脂肪酶 Novozym® 435 通过正丁醇与乙酸的酯化来研究乙酸丁酯的合成。由于该酯的酶促合成，乙酸和正丁醇的浓度必须受到限制这些试剂对酶造成的有害影响。研究了三种乙酸添加方法（单次、逐步和连续添加）以最小化酸浓度。当使用单一或逐步添加酸时，在较高浓度的试剂存在下观察到酸转化率显着下降。然而，使用连续添加乙酸的无溶剂系统有利于乙酸丁酯的合成，