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The design and characterization of a hypersensitive glucose sensor: two enzymes co-fixed on a copper phosphate skeleton.
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2019-11-25 , DOI: 10.1039/c9tb02294e
Ye Yuan 1 , Zhendong Fu 1 , Kai Wang 1 , Zhenyu Zhao 1 , Hui Li 1 , Zhi Wang 2 , Liping Wang 2
Affiliation  

In this study, we first reported a new glucose sensor for the preparation of a glucose oxidase (GOx)-conjugated novel artificial peroxidase mimic deuterohemin peptide (Dh-Ala-His-Thr-Val-Glu-Lys, DhHP-6) (GOx&DhHP-6–Cu3(PO4)2) in order to determine the glucose concentration. The same catalytic environment (pH 7.0, 35 °C) between glucose oxidase (GOx) and DhHP-6 ensured their utilization in catalytic cascade reactions. DhHP-6 and GOx were co-fixed on a copper phosphate skeleton, which ensured resistance toward harsh environments and improved storage stability. Meanwhile, the sensor reduced the diffusion of hydrogen peroxide (H2O2) in solutions by immobilization. GOx&DhHP-6–Cu3(PO4)2 possessed a wide linear range of 5–2000 μM, and a limit of detection of 0.5 μM. Based on the above-mentioned results, a new method to rapidly detect glucose concentrations was successfully developed using GOx&DhHP-6–Cu3(PO4)2 nanoflowers with high sensitivity and an easily applied methodology.

中文翻译:

超敏葡萄糖传感器的设计和表征:两种酶共固定在磷酸铜骨架上。

在这项研究中,我们首先报道了一种新型葡萄糖传感器,用于制备偶联有葡萄糖氧化酶(GOx)的新型人造过氧化物酶模拟去铁血红素肽(Dh-Ala-His-Thr-Val-Glu-Lys,DhHP-6)(GOx&DhHP -6–Cu 3(PO 42)以确定葡萄糖浓度。葡萄糖氧化酶(GOx)和DhHP-6之间的催化环境相同(pH 7.0,35°C)确保了它们在催化级联反应中的利用。DhHP-6和GOx共同固定在磷酸铜骨架上,从而确保了对恶劣环境的抵抗力并提高了存储稳定性。同时,传感器通过固定减少了溶液中过氧化氢(H 2 O 2)的扩散。GOx&DhHP-6–Cu 3(PO4 2的线性范围为5–2000μM,检出限为0.5μM。基于上述结果,使用高灵敏度的GOx&DhHP-6-Cu 3(PO 4 2纳米花成功开发了一种快速检测葡萄糖浓度的新方法,该方法简便易行。
更新日期:2019-11-25
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