Mitochondrial function has been predominantly measured ex vivo. Due to isolation and preservation procedures ex vivo measurements might misrepresent in vivo mitochondrial conditions. Direct measurement of in vivo mitochondrial oxygen tension (mitoPO2) and oxygen disappearance rate (ODR) with the protoporphyrin IX-triplet state lifetime technique (PpIX-TSLT) might increase our understanding of mitochondrial dysfunction in the pathophysiology of acute disease. LPS administration decreased mitochondrial respiration (ODR) in vivo but did not alter mitochondrial function as assessed with ex vivo techniques (high resolution respirometry and specific complex determinations). PpIX-TSLT measures in vivo mitoPO2 and ODR and can be applied non-invasively at the skin.

中文翻译：

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大鼠内毒素血症模型中线粒体功能的非侵入性与离体测量：对线粒体治疗的监测

线粒体功能主要是在体外测量的。由于分离和保存程序，离体测量可能会歪曲体内线粒体条件。使用原卟啉 IX-三重态寿命技术 (PpIX-TSLT) 直接测量体内线粒体氧张力 (mitoPO2) 和氧消失率 (ODR) 可能会增加我们对急性疾病病理生理学中线粒体功能障碍的理解。LPS 给药在体内降低了线粒体呼吸 (ODR)，但没有改变线粒体功能，如用离体技术（高分辨率呼​​吸测定法和特定复杂测定）评估的那样。PpIX-TSLT 测量体内 mitoPO2 和 ODR，并且可以非侵入性地应用于皮肤。