The black soldier fly (BSF), Hermetia illucens (Diptera: Stratiomyidae), is renowned for its bioconversion of organic waste into a sustainable source of animal feed. We report a high-quality genome of 1.1 Gb and a consensus set of 16,770 gene models for this beneficial species. Compared to those of other dipteran species, the BSF genome has undergone a substantial expansion in functional modules related to septic adaptation, including immune system factors, olfactory receptors, and cytochrome P450s. We further profiled midgut transcriptomes and associated microbiomes of BSF larvae fed with representative types of organic waste. We find that the pathways related to digestive system and fighting infection are commonly enriched and that Firmicutes bacteria dominate the microbial community in BSF across all diets. To extend its potential practical applications, we further developed an efficient CRISPR/Cas9-based gene editing approach and implemented this to yield flightless and enhanced feeding capacity phenotypes, both of which could expand BSF production capabilities. Our study provides valuable genomic and technical resources for optimizing BSF lines for industrialization.

中文翻译：

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天然废物回收者黑水虻的基因组景观和基因操纵。


黑水虻 (BSF)、Hermetia illucens（双翅目：Stratiomyidae）以其将有机废物生物转化为可持续动物饲料来源而闻名。我们报告了该有益物种的 1.1 Gb 高质量基因组和 16,770 个基因模型的共识集。与其他双翅目物种相比，BSF 基因组在与脓毒症适应相关的功能模块上经历了大幅扩展，包括免疫系统因子、嗅觉受体和细胞色素 P450。我们进一步分析了饲喂代表性类型有机废物的 BSF 幼虫的中肠转录组和相关微生物组。我们发现，与消化系统和抗感染相关的途径普遍丰富，并且厚壁菌门在所有饮食的 BSF 微生物群落中占主导地位。为了扩展其潜在的实际应用，我们进一步开发了一种高效的基于 CRISPR/Cas9 的基因编辑方法，并实施该方法来产生不会飞的和增强的摄食能力表型，这两者都可以扩大 BSF 的生产能力。我们的研究为优化 BSF 品系以实现工业化提供了宝贵的基因组和技术资源。