The degradation rate of polyester scaffolds has been emphasised as one of the main areas of concern in bone tissue engineering. In ideal conditions, the degradation of polymeric constructs should match regeneration of the injured tissue. Thus, there is an imperative need to strictly define and understand determinants influencing the degradation rate of scaffolds. In this study, we focused on the effect of filament shift introduction on degradation behaviour of the polymeric-based scaffolds. The poly(l-lactide-co-glycolide) (PLGA), poly(l-lactide-co-ε-caprolactone) (PLCL) and their tricalcium-phosphate-loaded (TCP) composites containing 20 and 40 wt% of filler, were utilized to fabricate constructs using modified fused deposition modeling (FDM). The scaffolds were designed with filament lay-down pattern of 0°/90° and with or without the modifications of filament distance in n+2 layer, shifted and non-shifted constructs were obtained, respectively. To investigate the degradation profile, the change of mass, pH, water absorption and initial molecular weight (M_w0) loss was observed during the degradation study in phosphate buffered saline (PBS) at 37 °C for up to 48 weeks. The scaffold morphology was evaluated utilizing scanning electron microscopy (SEM) and the visualization of the topography was performed utilizing atomic force microscopy (AFM). Surface area to volume ratio (SVR) and porosity were determined using micro-computed tomography (μCT). The fluid flow simulations were used to define the permeability of two investigated groups of scaffolds. The results of this study clearly demonstrate the accelerating effect of filament shift introduction on degradation behaviour in the scaffolds with similar porosity and SVR. The decrease of M_w0 was significantly higher in case of all shifted samples. We assume that faster degradation of shifted constructs may be attributed to their tortuosity, making them less permeable and prone to the degradation, as the result of the accumulation of acidic products in the tortuous architecture of the samples. Thus, the effect of introduction of filament shift into scaffold architecture comprise an attractive approach to influence the degradation rate in case of bone regeneration with the use of polyesters scaffolds.

聚酯支架的降解速率已被强调为骨组织工程中关注的主要领域之一。在理想条件下，聚合物构建体的降解应与受伤组织的再生相匹配。因此，迫切需要严格定义和理解影响支架降解速率的决定因素。在这项研究中，我们集中于引入细丝移位对基于聚合物的支架的降解行为的影响。聚（升丙交酯-共-乙交酯）（PLGA），聚（升丙交酯-共- ε-己内酯）（PLCL）及其负载了20％和40％重量的磷酸三钙的（TCP）复合材料被用于通过改进的熔融沉积建模（FDM）来制造构建体。将支架设计为具有0°/ 90°的细丝铺放模式，并在n + 2层中修改或不修改细丝距离，分别获得了移位和不移位的构建体。研究降解曲线，质量，pH，吸水率和初始分子量的变化（M _w0）在磷酸盐缓冲液（PBS）中于37°C进行长达48周的降解研究期间观察到损失。使用扫描电子显微镜（SEM）评估支架的形态，并使用原子力显微镜（AFM）进行形貌的可视化。表面积/体积比（SVR）和孔隙率使用微型计算机断层扫描（μCT）确定。流体流动模拟被用来定义两组研究的支架的渗透性。这项研究的结果清楚地证明了引入细丝移位对具有相似孔隙率和SVR的脚手架的降解行为的加速作用。M _w0的减少在所有移位样本的情况下均显着更高。我们假设，由于酸性产品在样品的曲折结构中积累，移位后的构建体的更快降解可能归因于它们的曲折性，使其渗透性更差并且更易于降解。因此，在使用聚酯支架进行骨再生的情况下，将细丝移位引入到支架结构中的效果包括一种有吸引力的方法来影响降解速率。