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Diffusion behavior of cerebral metabolites of congenital portal systemic shunt mice assessed noninvasively by diffusion-weighted 1 H magnetic resonance spectroscopy.
NMR in Biomedicine ( IF 2.7 ) Pub Date : 2019-11-25 , DOI: 10.1002/nbm.4198 Masoumeh Dehghani 1, 2 , Nicolas Kunz 3 , Hongxia Lei 3
NMR in Biomedicine ( IF 2.7 ) Pub Date : 2019-11-25 , DOI: 10.1002/nbm.4198 Masoumeh Dehghani 1, 2 , Nicolas Kunz 3 , Hongxia Lei 3
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Diffusion-weighted 1 H-MRS (DW-MRS) allows for noninvasive investigation of the cellular compartmentalization of cerebral metabolites. DW-MRS applied to the congenital portal systemic shunt (PSS) mouse brain may provide specific insight into alterations of cellular restrictions associated with PSS in humans. At 14.1 T, adult male PSS and their age-matched healthy (Ctrl) mice were studied using DW-MRS covering b-values ranging from 0 to 45 ms/μm2 to determine the diffusion behavior of abundant metabolites. The remarkable sensitivity and spectral resolution, in combination with very high diffusion weighting, allowed for precise measurement of the diffusion properties of endogenous N-acetyl-aspartate, total creatine, myo-inositol, total choline with extension to glutamine and glutamate in mouse brains, in vivo. Most metabolites had comparable diffusion properties in PSS and Ctrl mice, suggesting that intracellular distribution space for these metabolites was not affected in the model. The slightly different diffusivity of the slow decaying component of taurine (0.015 ± 0.003 μm2 /ms in PSS vs 0.021 ± 0.002 μm2 /ms in Ctrl, P < 0.05) might support a cellular redistribution of taurine in the PSS mouse brain.
中文翻译：
先天性门静脉系统分流小鼠脑代谢产物的扩散行为通过扩散加权1 H磁共振波谱进行无创评估。
扩散加权1 H-MRS（DW-MRS）允许对脑代谢物的细胞区室化进行非侵入性研究。应用于先天性门静脉系统分流（PSS）小鼠大脑的DW-MRS可能为人类中与PSS相关的细胞限制改变提供特定见解。在14.1 T时，使用DW-MRS研究了成年雄性PSS及其年龄匹配的健康（Ctrl）小鼠，其b值介于0到45 ms /μm2之间，以确定丰富的代谢物的扩散行为。出色的灵敏度和光谱分辨率，加上非常高的扩散权重，可以精确测量内源性N-乙酰天门冬氨酸，总肌酸，肌醇，总胆碱以及谷氨酰胺和谷氨酸在小鼠脑中的扩散特性，体内。大多数代谢物在PSS和Ctrl小鼠中具有可比的扩散特性，表明这些代谢物的细胞内分布空间在模型中不受影响。牛磺酸的缓慢衰减成分的扩散率略有不同（PSS中为0.015±0.003μm2/ ms，而Ctrl中为0.021±0.002μm2/ ms，P <0.05）可能支持牛磺酸在PSS小鼠脑中的细胞再分布。
更新日期：2020-01-21
中文翻译：
先天性门静脉系统分流小鼠脑代谢产物的扩散行为通过扩散加权1 H磁共振波谱进行无创评估。
扩散加权1 H-MRS（DW-MRS）允许对脑代谢物的细胞区室化进行非侵入性研究。应用于先天性门静脉系统分流（PSS）小鼠大脑的DW-MRS可能为人类中与PSS相关的细胞限制改变提供特定见解。在14.1 T时，使用DW-MRS研究了成年雄性PSS及其年龄匹配的健康（Ctrl）小鼠，其b值介于0到45 ms /μm2之间，以确定丰富的代谢物的扩散行为。出色的灵敏度和光谱分辨率，加上非常高的扩散权重，可以精确测量内源性N-乙酰天门冬氨酸，总肌酸，肌醇，总胆碱以及谷氨酰胺和谷氨酸在小鼠脑中的扩散特性，体内。大多数代谢物在PSS和Ctrl小鼠中具有可比的扩散特性，表明这些代谢物的细胞内分布空间在模型中不受影响。牛磺酸的缓慢衰减成分的扩散率略有不同（PSS中为0.015±0.003μm2/ ms，而Ctrl中为0.021±0.002μm2/ ms，P <0.05）可能支持牛磺酸在PSS小鼠脑中的细胞再分布。
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