Tunable Adhesion of Different Cell Types Modulated by Thermoresponsive Polymer Brush Thickness.,Biomacromolecules

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Tunable Adhesion of Different Cell Types Modulated by Thermoresponsive Polymer Brush Thickness.
Biomacromolecules ( IF 5.5 ) Pub Date : 2019-12-06 , DOI: 10.1021/acs.biomac.9b01437
Jiamin Lian _{1,

2} , Haifeng Xu _{1,

2} , Shun Duan _{1,

2} , Xuejia Ding _{1,

2} , Yang Hu _{1,

2} , Nana Zhao _{1,

2} , Xiaokang Ding _{1,

2} , Fu-Jian Xu _{1,

2}

Affiliation

Tunable adhesion of different cell types on well-defined surfaces has attracted common interests in the field of biomaterial science and surface engineering. Herein, we demonstrate a new strategy for the regulation of cell adhesion by simply controlling the thickness of thermoresponsive poly(N-isopropylacrylamide) (PNIPAAm) brushes via surface-initiated atom transfer radical polymerization (ATRP). The adhesion of different cell types (4T1, HEK293, H9C2, HUVEC, and L929) can be easily modulated by varying the thickness of PNIPAAm brushes from 5.9 ± 1.0 nm (PN1) to 69.0 ± 5.0 nm (PN6). The fluorescent staining of different cell types on a variety of surfaces reveals that the thickness of PNIPAAm brushes would regulate the assembly of F-actin and the expression of vinculin and fibronectin, which are essential in regulating the adherent status of cells. Moreover, the cellular morphologies revealed that the adherent cells are well-spread, and multiple pseudopod extensions and protrusions can be observed at the margin of cells. This work provides a facile strategy for regulating tunable adhesion of different cell types, which may find applications in tissue engineering and regenerative medicine.

中文翻译：

由热敏性聚合物刷厚度调节的不同类型电池的可调粘附力。

在明确的表面上，不同细胞类型的可调节粘附力已在生物材料科学和表面工程领域引起了共同的兴趣。在这里，我们展示了一种通过简单地通过表面引发的原子转移自由基聚合（ATRP）控制热响应性聚（N-异丙基丙烯酰胺）（PNIPAAm）刷子的厚度来调节细胞粘附的新策略。通过将PNIPAAm笔刷的厚度从5.9±1.0 nm（PN1）更改为69.0±5.0 nm（PN6），可以轻松调节不同细胞类型（4T1，HEK293，H9C2，HUVEC和L929）的粘附力。在各种表面上对不同类型细胞的荧光染色表明，PNIPAAm刷的厚度会调节F-肌动蛋白的装配以及纽蛋白和纤连蛋白的表达，这对于调节细胞的粘附状态至关重要。此外，细胞形态揭示了贴壁细胞分布良好，并且在细胞边缘可以观察到多个假足的延伸和突出。这项工作为调节不同细胞类型的可调粘附提供了一种简便的策略，这可能在组织工程和再生医学中得到应用。

更新日期：2019-12-07

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