Non-invasive sonodynamic therapy (SDT) was developed because of its advantages of high penetration depth and low side effects; however, tumor hypoxia greatly restricts its therapeutic effect. In this study, we aimed to develop ideal O₂ self-supplementing nanoparticles for imaging-guided enhanced sonodynamic therapy of tumors with the adept coalescence of biology with nanotechnology.

Methods: Based on the natural enzyme system of red blood cells (RBC), biomimetic nanoparticles (QD@P)Rs were fabricated by encapsulating Ag₂S quantum dots (QD) in RBC vesicle membranes. The anti-tumor drug PEITC was employed to increase the intracellular H₂O₂ concentration in tumor cells.

Results: In vitro and in vivo experiments demonstrated excellent biocompatibility and prolonged blood circulation of (QD@P)Rs. Following oral administration of PEITC in mice to improve the H₂O₂ concentration, the enzyme in the nanoprobe catalyzed endogenous H₂O₂ to increase O₂ content and effectively alleviate tumor hypoxia. Triggered by ultrasound under the guidance of fluorescence imaging, (QD@P)Rs generated reactive oxygen species (ROS) to induce tumor cell death, and the increased content of O₂ significantly enhanced the effect of SDT.

Conclusion: Ag₂S QDs were used, for the first time, as a sonosensitizer in the SDT field. In this study, we integrated the advantages of the natural enzyme system and SDT to develop a novel approach for effective non-invasive treatment of cancer.

由于其具有高穿透深度和低副作用的优点，因此开发了无创声动力疗法（SDT）。然而，肿瘤缺氧极大地限制了其治疗效果。在这项研究中，我们旨在开发理想的O ₂自补充纳米颗粒，以借助纳米技术与生物相结合来对肿瘤进行影像引导的增强声波动力疗法。

方法：基于红细胞（RBC）的天然酶体系，通过将Ag ₂ S量子点（QD）包裹在RBC囊泡膜中来制备仿生纳米颗粒（QD @ P）Rs 。使用抗肿瘤药物PEITC来增加肿瘤细胞中细胞内H ₂ O _2的浓度。

结果：体外和体内实验证明（QD @ P）Rs具有出色的生物相容性和延长的血液循环。在给小鼠口服PEITC以提高H ₂ O ₂浓度后，纳米探针中的酶催化内源性H ₂ O ₂来增加O ₂含量并有效缓解肿瘤缺氧。（QD @ P）Rs由超声触发，在荧光成像的指导下，产生活性氧（ROS）诱导肿瘤细胞死亡，O ₂含量的增加显着增强了SDT的作用。

结论： Ag ₂ S量子点首次在SDT领域用作声敏剂。在这项研究中，我们综合了天然酶系统和SDT的优势，以开发出一种有效的无创治疗癌症的新方法。