Endothelialization is of great significance for vascular remodeling, as well as for the success of implanted vascular grafts/stents in cardiovascular disease treatment. However, desirable endothelialization on synthetic biomaterials remains greatly challenging owing to extreme difficulty in offering dynamic guidance on endothelial cell (EC) functions resembling the native extracellular matrix (ECM)-mediated effects. Here, we demonstrate a bilayer platform with near infrared (NIR)-triggered transformable topographies, which can alter the geometries and functions of human ECs by tunable topographical cues in a remote-controlled manner, yet bring no damage on cell viability. The migration and the adhesion/spreading of human ECs are respectively promoted by the temporary anisotropic and permanent isotropic topographies of the platform in turn, which appropriately meet the requirements of stage-specific EC manipulation for endothelialization. In addition to the potential of promoting the development of new generation of vascular grafts/stents enabling rapid endothelialization, this stage-specific cell manipulation platform also holds promise in various biomedical fields since the needs of stepwise control over different cell functions are common in wound healing and various tissue regeneration processes.

中文翻译：

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用于按需诱导内皮化的阶段特异性细胞操作平台

内皮化对于血管重塑以及植入血管移植物/支架在心血管疾病治疗中的成功具有重要意义。然而，由于在提供类似于天然细胞外基质 (ECM) 介导效应的内皮细胞 (EC) 功能的动态指导方面极其困难，因此合成生物材料上的理想内皮化仍然具有很大的挑战性。在这里，我们展示了一个具有近红外 (NIR) 触发的可变形拓扑结构的双层平台，它可以通过远程控制的可调拓扑线索改变人类 ECs 的几何形状和功能，但不会对细胞活力造成损害。平台的临时各向异性和永久各向同性地形依次分别促进了人类内皮细胞的迁移和粘附/扩散，适当地满足阶段特定 EC 操作对内皮化的要求。除了促进新一代血管移植物/支架的发展以实现快速内皮化之外，这个阶段特定的细胞操作平台在各种生物医学领域也很有前景，因为在伤口愈合中需要逐步控制不同的细胞功能以及各种组织再生过程。