The endolysosomal system is critical for protein homeostasis in cells. A common way of studying protein transport and degradation (e.g. via autophagy) is by labeling vesicular structures such as endosomes, autophagosomes, lysosomes, or model substrates with fluorescent tags or by fluorescent antibody staining. Detailed analyses require quantification of hundreds of structures under various conditions. Typically, the images are analyzed individually with software such as the widely available Fiji/ImageJ (https://imagej.net/Fiji/Downloads), adjusting and thresholding each image and channel independently, which is a very labor intensive and fastidious task. To streamline the process, we developed a plug-in that, integrated into Fiji, enables the automated quantification of vesicular (i.e. punctate) structures. Importantly, the process still allows the operator to evaluate and have control over all the phases of quantification process.

Abbreviations

CMA: chaperone-mediated autophagy; CSV: comma separated values; eMI: endosomal microautophagy; Fiji: Fiji is just ImageJ; MA: macroautophagy; SParQ: Streamlined Particle Quantification

中文翻译：

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流线型粒子量化 (SParQ) 插件是一种自动荧光囊泡量化插件，用于 Fiji/ImageJ 中的粒子量化。

内溶酶体系统对于细胞中的蛋白质稳态至关重要。研究蛋白质转运和降解（例如通过自噬）的常用方法是用荧光标签或荧光抗体染色标记囊泡结构，例如内体、自噬体、溶酶体或模型底物。详细分析需要在各种条件下对数百种结构进行量化。通常，图像是使用软件单独分析的，例如广泛使用的 Fiji/ImageJ (https://imagej.net/Fiji/Downloads)，独立调整和阈值每个图像和通道，这是一项非常劳动密集型和繁琐的任务。为了简化流程，我们开发了一个插件，集成到斐济，能够自动量化囊泡（即点状）结构。重要的，

缩写

CMA：伴侣介导的自噬；CSV：逗号分隔值；eMI：内体微自噬；斐济：斐济只是 ImageJ；MA：巨自噬；SParQ：流线型粒子量化