An old oak tree (Quercus robur), colonized by two different lineages of the Cryptococcus neoformans species complex (VNI and VNIV), was investigated to determine the distribution of cryptococcal yeasts in the different micro-habitats of the tree. The yeasts were isolated from most of the tree micro-habitats such as bark, trunk cavities, soil beneath the tree, arthropods living on the tree, and air. Sunlight exposure and low temperatures decreased in vitro growth of VNI isolates but not that of VNIV ones. Both VNI and VNIV strains isolated from the tree were able to produce basidiospores by mating assays. The investigated tree represents an environmental reservoir of C. neoformans, where it is able to survive and probably to reproduce, and from where blastospores and basidiospores, exposed to wind, water and contact with arthropods, birds, and mammals, may be dispersed in the environment as potential infectious propagules.

研究了由两种新隐球菌物种复合体（VNI和VNIV）定居的老橡树（栎栎），以确定隐球菌酵母在树的不同微生境中的分布。酵母是从大多数树的微生境中分离出来的，例如树皮，树干腔，树下的土壤，生活在树上的节肢动物和空气。日光照射和低温会降低VNI分离株的体外生长，但不会降低VNIV分离株的体外生长。从树中分离出的VNI和VNIV菌株均能够通过交配试验产生担子孢子。被调查的树代表了新孢梭菌的环境储藏库， 它能够生存并可能繁殖的地方，以及暴露于风，水和与节肢动物，鸟类和哺乳动物接触的胚状孢子和担子孢子可能会作为潜在的传染性繁殖物散布在环境中。