Protective effect of Hedansanqi Tiaozhi Tang against non-alcoholic fatty liver disease in vitro and in vivo through activating Nrf2/HO-1 antioxidant signaling pathway.,Phytomedicine

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Protective effect of Hedansanqi Tiaozhi Tang against non-alcoholic fatty liver disease in vitro and in vivo through activating Nrf2/HO-1 antioxidant signaling pathway.
Phytomedicine ( IF 6.7 ) Pub Date : 2019-11-22 , DOI: 10.1016/j.phymed.2019.153140
Mingyang Qiu ₁ , Fengqin Xiao ₁ , Tianning Wang ₁ , Songlan Piao ₂ , Wu Zhao ₁ , Shuai Shao ₁ , Mingming Yan ₃ , Daqing Zhao ₄

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BACKGROUND Hedansanqi Tiaozhi Tang extract (HTT) consists of Notoginseng, Danshen, Hawthorn and Lotus leaf from traditional Chinese medicine, which has significant therapeutic effects on hyperlipidemia in patients with non-alcoholic fatty liver disease (NAFLD). PURPOSE This study sought to evaluate the pharmacological effects and molecular mechanism of HTT for the treatment of hyperlipidemia in adipocytes and animal model with NAFLD. METHODS Quantitative phytochemical analysis of HTT was performed by HPLC. Antioxidant activity and the adipogenesis in 3T3-L1 cells were assessed. In the rat model induced by high-fat diet, lipid-related and antioxidant markers in serum and liver were detected. Moreover, the organ weights, non-alcoholic steatohepatitis (NASH) score and the levels of Nrf2 and HO-1 in liver sections were analyzed by tissue pathological techniques. RESULTS 8 constituents were identified in HTT including saponins, flavonoids, alkaloids and others. HTT treatment enhanced antioxidant activities and promoted lipolysis in 3T3-L1 adipocytes. We also found that HTT inhibited weight gain, reduced the lipid profiles and improved the liver function and pathological characteristics induced by high-fat diet. In addition, HTT activated the Nrf2/HO-1 antioxidant pathway in the liver. CONCLUSION HTT has protective effect against NAFLD in vitro and in vivo by activating the Nrf2/HO-1 antioxidant pathway.

中文翻译：

荷丹三七调脂汤通过激活Nrf2 / HO-1抗氧化剂信号通路，在体内外对非酒精性脂肪肝的保护作用。

背景技术和丹三七调脂汤提取物（HTT）由三七，丹参，山楂和荷叶组成，对非酒精性脂肪肝（NAFLD）患者的高脂血症具有显着的治疗作用。目的本研究试图评估HTT在脂肪细胞和NAFLD动物模型中治疗高脂血症的药理作用和分子机制。方法采用高效液相色谱法对HTT进行定量植物化学分析。评估了3T3-L1细胞的抗氧化活性和脂肪形成。在高脂饮食诱导的大鼠模型中，检测到血清和肝脏中脂质相关和抗氧化剂标记。而且器官的重量通过组织病理学技术分析非酒精性脂肪性肝炎（NASH）评分以及肝脏切片中Nrf2和HO-1的水平。结果在HTT中鉴定出8种成分，包括皂苷，类黄酮，生物碱等。HTT处理可增强3T3-L1脂肪细胞的抗氧化活性并促进脂肪分解。我们还发现，HTT可以抑制体重增加，减少脂质分布并改善高脂饮食诱导的肝功能和病理特征。此外，HTT还激活了肝脏中的Nrf2 / HO-1抗氧化剂途径。结论HTT通过激活Nrf2 / HO-1抗氧化剂途径在体内外对NAFLD具有保护作用。HTT处理可增强3T3-L1脂肪细胞的抗氧化活性并促进脂解作用。我们还发现，HTT可以抑制体重增加，减少脂质分布并改善高脂饮食诱导的肝功能和病理特征。此外，HTT还激活了肝脏中的Nrf2 / HO-1抗氧化剂途径。结论HTT通过激活Nrf2 / HO-1抗氧化剂途径在体内外对NAFLD具有保护作用。HTT处理可增强3T3-L1脂肪细胞的抗氧化活性并促进脂肪分解。我们还发现，HTT可以抑制体重增加，减少脂质分布并改善高脂饮食诱导的肝功能和病理特征。此外，HTT还激活了肝脏中的Nrf2 / HO-1抗氧化剂途径。结论HTT通过激活Nrf2 / HO-1抗氧化剂途径在体内外对NAFLD具有保护作用。

更新日期：2019-11-22

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