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CcOBP2 plays a crucial role in 3-carene olfactory response of the parasitoid wasp Chouioia cunea.
Insect Biochemistry and Molecular Biology ( IF 3.2 ) Pub Date : 2019-11-22 , DOI: 10.1016/j.ibmb.2019.103286 Lina Pan 1 , Weifang Xiang 2 , Zeyang Sun 1 , Yixin Yang 1 , Jiayi Han 1 , Yonghui Wang 1 , Chuncai Yan 1 , Min Li 1
Insect Biochemistry and Molecular Biology ( IF 3.2 ) Pub Date : 2019-11-22 , DOI: 10.1016/j.ibmb.2019.103286 Lina Pan 1 , Weifang Xiang 2 , Zeyang Sun 1 , Yixin Yang 1 , Jiayi Han 1 , Yonghui Wang 1 , Chuncai Yan 1 , Min Li 1
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Chouioia cunea (Yang) is a pupal parasitoid wasp and this species is able to seek host insects depending on its olfactory system. However, the molecular mechanism of the olfactory system in the C. cunea is still limited. To identify putative semiochemicals bound to CcOBP2, a protein specifically expressed in antennae, 14 compounds from the pupae of H. cunea and 11 common volatile compounds from plants were selected for competitive fluorescence binding assay. The result of the binding assay showed that five compounds were able to bind toCcOBP2. The electroantennogram (EAG) demonstrated that the antennae had a significant response to the 3-Carene, a bicyclic monoterpene, and C. cunea could be obviously attracted by this compound. The behavioral response to 3- carene was dramatically weakened when CcOBP2 was specifically knocked down. The molecular docking result indicated that several amino acids especially Ile-81, Val-122, Phe-123 of CcOBP2 were responsible for binding to 3-Carene. Furthermore, there was a repellent effect on the host H. cunea with the treatment of the 3-Carene. This study illustrated that CcOBP2 might be a crucial protein involved in the olfactory signaling pathway and the 3-Carene, secreted from plants, could probably have a potential role in repelling pests as well as attracting natural enemies.
中文翻译：
CcOBP2在寄生蜂黄蜂Chouioia cunea的3-烯属嗅觉反应中起着至关重要的作用。
Chouioia cunea（Yang）是一种para寄生的黄蜂，该物种能够根据其嗅觉系统寻找寄主昆虫。但是，C。cunea嗅觉系统的分子机制仍然受到限制。为了鉴定与CcOBP2（一种在触角中特异性表达的蛋白质）结合的推定的化学信息素，选择了14种来自中华稻H的化合物和11种来自植物的常见挥发性化合物进行竞争性荧光结合测定。结合测定的结果表明五种化合物能够结合到CcOBP2。脑电图（EAG）表明触角对3-碳烯，双环单萜具有显着的响应，该化合物显然吸引了C. cunea。当专门敲低CcOBP2时，对3-carene的行为反应会大大减弱。分子对接结果表明，CcOBP2的几种氨基酸，特别是Ile-81，Val-122，Phe-123负责与3-Carene的结合。此外，用3-Carene处理对寄主H. cunea有驱避作用。这项研究表明，CcOBP2可能是嗅觉信号通路中的关键蛋白，植物分泌的3-Carene可能具有驱除害虫和吸引天敌的潜在作用。
更新日期：2019-11-22
中文翻译：
CcOBP2在寄生蜂黄蜂Chouioia cunea的3-烯属嗅觉反应中起着至关重要的作用。
Chouioia cunea（Yang）是一种para寄生的黄蜂，该物种能够根据其嗅觉系统寻找寄主昆虫。但是，C。cunea嗅觉系统的分子机制仍然受到限制。为了鉴定与CcOBP2（一种在触角中特异性表达的蛋白质）结合的推定的化学信息素，选择了14种来自中华稻H的化合物和11种来自植物的常见挥发性化合物进行竞争性荧光结合测定。结合测定的结果表明五种化合物能够结合到CcOBP2。脑电图（EAG）表明触角对3-碳烯，双环单萜具有显着的响应，该化合物显然吸引了C. cunea。当专门敲低CcOBP2时，对3-carene的行为反应会大大减弱。分子对接结果表明，CcOBP2的几种氨基酸，特别是Ile-81，Val-122，Phe-123负责与3-Carene的结合。此外，用3-Carene处理对寄主H. cunea有驱避作用。这项研究表明，CcOBP2可能是嗅觉信号通路中的关键蛋白，植物分泌的3-Carene可能具有驱除害虫和吸引天敌的潜在作用。
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