CRISPR-mediated genome editing is a revolutionary technology for genome manipulation that uses the CRISPR-Cas systems and base editors. Currently, poor efficiency and off-target problems have impeded the application of CRISPR systems. The on-target efficiency has been improved in several advanced versions of CRISPR systems, whereas the off-target detection still remains a key challenge. Here, we outline the different versions of CRISPR systems and off-target detection strategies, discuss the merits and limitations of off-target detection methods, and provide potential implications for further gene editing research.

中文翻译：

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检测和减少 CRISPR 介导的基因组编辑产生的脱靶效应的进展。

CRISPR 介导的基因组编辑是一项革命性的基因组操作技术，它使用 CRISPR-Cas 系统和碱基编辑器。目前，效率低下和脱靶问题阻碍了CRISPR系统的应用。多个先进版本的 CRISPR 系统的靶向效率得到了提高，但脱靶检测仍然是一个关键挑战。在这里，我们概述了不同版本的 CRISPR 系统和脱靶检测策略，讨论了脱靶检测方法的优点和局限性，并为进一步的基因编辑研究提供了潜在的启示。