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Pd-ligand 1 is expressed in inflammatory cells but not in neoplastic cells in hepatocellular carcinoma: An immunohistochemical study.
Acta Histochemica ( IF 2.3 ) Pub Date : 2019-11-22 , DOI: 10.1016/j.acthis.2019.151468
Francesco Vasuri 1 , Azzurra Nerpiti 1 , Stefano Zagnoni 1 , Matteo Ravaioli 2 , Antonia D'Errico 1 , Michelangelo Fiorentino 1
Affiliation  

Nowadays, the major limit to the immunohistochemical (IHC) analysis of tissue PD-L1 is the high variability of the monoclonal antibodies commercially available. Aims of the present paper are to assess the best clone and the most suitable scoring for PD-L1 IHC determination on human hepatocellular carcinoma (HCC) among three commercially available clones, and to evaluate which PD-L1 clone is the best in predicting HCC aggressiveness in vivo. We built a tissue microarray (TMA) with 60 retrospective HCC cases, including the correspondent non-tumoral tissue. IHC was automatically performed using the following anti-PD-L1 clones: 28.8, SP142, and SP263. As results, we did not find any immunoreactivity for PD-L1 in both neoplastic and normal hepatocytes included in the TMA using the three antibodies. Positivity for PD-L1 was exclusively seen in inflammatory cells within the HCC tissue and in cirrhotic parenchyma. When a gold standard was assessed, the sensitivity of SP142, 28.8 and SP263 was 46 %, 54 % and 85 % respectively. Using the SP263 clone, the absolute number of PD-L1-positive inflammatory cells in the HCC cores was paired with the number of PD-L1-positive inflammatory cells in the corresponding non-tumoral tissue (P = 0.001). Finally, using SP263, the mean number of PD-L1-positive cells was 11.3 ± 12.6 in HCC from deceased patients, versus 4.7 ± 5.2 in alive patients (p = 0.039). SP263 is the most sensitive clone for PD-L1 IHC tissue determination in HCC, as well as the best antibody for the assessment of its biological behavior.

中文翻译:

免疫细胞化学研究表明,Pd-配体1在肝细胞癌的炎症细胞中表达,而在肿瘤细胞中不表达。

如今,对组织PD-L1的免疫组织化学(IHC)分析的主要限制是市售单克隆抗体的高变异性。本文的目的是评估三种市售克隆中用于人肝细胞癌(HCC)的PD-L1 IHC测定的最佳克隆和最合适的评分,并评估哪种PD-L1克隆最能预测HCC侵袭性体内。我们建立了60例回顾性HCC病例的组织微阵列(TMA),包括相应的非肿瘤组织。使用以下抗PD-L1克隆自动执行IHC:28.8,SP142和SP263。结果,我们在使用这三种抗体的TMA所包括的赘生性和正常肝细胞中均未发现针对PD-L1的任何免疫反应。PD-L1的阳性仅在HCC组织内的炎症细胞和肝硬化实质中可见。评估金标准时,SP142、28.8和SP263的灵敏度分别为46%,54%和85%。使用SP263克隆,将HCC核心中PD-L1阳性炎性细胞的绝对数量与相应的非肿瘤组织中PD-L1阳性炎性细胞的数量配对(P = 0.001)。最后,使用SP263,死者肝癌中PD-L1阳性细胞的平均数为11.3±12.6,而活着患者则为4.7±5.2(p = 0.039)。SP263是用于HCC中PD-L1 IHC组织测定的最敏感克隆,也是评估其生物学行为的最佳抗体。SP142、28.8和SP263的灵敏度分别为46%,54%和85%。使用SP263克隆,将HCC核心中PD-L1阳性炎性细胞的绝对数量与相应的非肿瘤组织中PD-L1阳性炎性细胞的数量配对(P = 0.001)。最后,使用SP263,死者肝癌中PD-L1阳性细胞的平均数为11.3±12.6,而活着患者则为4.7±5.2(p = 0.039)。SP263是用于HCC中PD-L1 IHC组织测定的最敏感克隆,也是评估其生物学行为的最佳抗体。SP142、28.8和SP263的灵敏度分别为46%,54%和85%。使用SP263克隆,将HCC核心中PD-L1阳性炎性细胞的绝对数量与相应的非肿瘤组织中PD-L1阳性炎性细胞的数量配对(P = 0.001)。最后,使用SP263,死者肝癌中PD-L1阳性细胞的平均数为11.3±12.6,而活着患者则为4.7±5.2(p = 0.039)。SP263是用于HCC中PD-L1 IHC组织测定的最敏感克隆,也是评估其生物学行为的最佳抗体。将HCC核心中PD-L1阳性炎症细胞的绝对数量与相应的非肿瘤组织中PD-L1阳性炎症细胞的数量配对(P = 0.001)。最后,使用SP263,死者肝癌中PD-L1阳性细胞的平均数为11.3±12.6,而活着患者则为4.7±5.2(p = 0.039)。SP263是用于HCC中PD-L1 IHC组织测定的最敏感克隆,也是评估其生物学行为的最佳抗体。将HCC核心中PD-L1阳性炎症细胞的绝对数量与相应的非肿瘤组织中PD-L1阳性炎症细胞的数量配对(P = 0.001)。最后,使用SP263,死者肝癌中PD-L1阳性细胞的平均数为11.3±12.6,而活着患者则为4.7±5.2(p = 0.039)。SP263是用于HCC中PD-L1 IHC组织测定的最敏感克隆,也是评估其生物学行为的最佳抗体。
更新日期:2019-11-22
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