Transcription Increases the Cooperativity of Ribonucleoprotein Assembly.,Cell

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Transcription Increases the Cooperativity of Ribonucleoprotein Assembly.
Cell ( IF 45.5 ) Pub Date : 2019-11-21 , DOI: 10.1016/j.cell.2019.11.007
Margaret L Rodgers ₁ , Sarah A Woodson ₁

Affiliation

The synthesis of new ribosomes begins during transcription of the rRNA and is widely assumed to follow an orderly 5' to 3' gradient. To visualize co-transcriptional assembly of ribosomal protein-RNA complexes in real time, we developed a single-molecule platform that simultaneously monitors transcription and protein association with the elongating transcript. Unexpectedly, the early assembly protein uS4 binds newly made pre-16S rRNA only transiently, likely due to non-native folding of the rRNA during transcription. Stable uS4 binding became more probable only in the presence of additional ribosomal proteins that bind upstream and downstream of protein uS4 by allowing productive assembly intermediates to form earlier. We propose that dynamic sampling of elongating RNA by multiple proteins overcomes heterogeneous RNA folding, preventing assembly bottlenecks and initiating assembly within the transcription time window. This may be a common feature of transcription-coupled RNP assembly.

中文翻译：

转录增加了核糖核糖核酸装配的协同作用。

新的核糖体的合成在rRNA的转录过程中开始，并且被广泛认为遵循有序的5'至3'梯度。为了实时观察核糖体蛋白-RNA复合物的共转录组装，我们开发了一个单分子平台，该平台可以同时监测转录和与延长转录本的蛋白质缔合。出乎意料的是，早期装配蛋白uS4仅短暂地结合新产生的16S之前的rRNA，这可能是由于rRNA在转录过程中发生了非天然折叠。只有在其他核糖体蛋白存在的情况下，稳定的uS4结合才能变得更可能，这些核糖体蛋白通过允许较早形成生产性组装中间体来结合蛋白uS4的上游和下游。我们建议通过多种蛋白质对延长RNA进行动态采样可以克服异源RNA折叠，防止程序集瓶颈，并在转录时间窗口内启动程序集。这可能是转录偶联RNP装配的共同特征。

更新日期：2019-11-22

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