Soybean [Glycine max (L.) Merr.] oil with high oleic acid (>75%) has increased oxidative stability and health benefits that are valuable for food, fuel, and industrial products. It has been determined that two naturally occurring mutations in genes FAD2‐1A and FAD2‐1B can combine to produce high oleic soybeans. The objective of this study was to test the effect of these mutant alleles on seed yield and oil and protein concentration. Molecular markers assisted in the creation of a population of 48 BC₃F_2:4 lines (93.75% expected genome commonality). Each line was classified into one of four genotypic groups where both FAD2‐1A and FAD2‐1B genes were either homozygous wild type or mutant, respectively. Twelve lines for each genotypic group were evaluated in three replications at six locations across Tennessee. There was no seed yield difference between the high oleic genotypic group and the other groups (P < 0.05). On the other hand, there were differences in fatty acid profiles and oil and protein concentrations. In combination, the mutant FAD2‐1A and FAD2‐1B alleles produced a mean of 803.1 g kg⁻¹ oleic acid. This is, on average, approximately 500 g kg⁻¹ more oleic acid compared to soybean lines with only one mutant FAD2‐1 allele. The high oleic double mutant group had more total oil (228.0 g kg⁻¹) and protein (401.0 g kg⁻¹) compared to all other genotypic groups (P < 0.05). Overall, this specific combination of mutant FAD2‐1A and FAD2‐1B alleles appears to generate conventional high oleic soybeans without a yield drag.

中文翻译：

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田纳西州具有突变FAD2-1A和FAD2-1B基因的高油大豆的田间表现

高油酸（> 75％）的大豆[ Glycine max（L.）Merr。]油具有增强的氧化稳定性和健康益处，对食品，燃料和工业产品具有重要意义。已经确定，基因FAD2-1A和FAD2-1B中的两个自然发生的突变可以结合产生高油酸大豆。这项研究的目的是测试这些突变等位基因对种子产量以及油和蛋白质浓度的影响。分子标记有助于创建48个BC ₃ F _2：4品系（预期的93.75％的基因组共有性）。每条品系均分为FAD2-1A和FAD2-1B四个基因型组之一这些基因分别是纯合野生型或突变型。在田纳西州的六个位置，以三个重复进行了每个基因型组的十二个品系的评估。高油酸基因型组与其他组之间没有种子产量差异（P  <0.05）。另一方面，脂肪酸谱以及油和蛋白质浓度存在差异。结合起来，突变的FAD2-1A和FAD2-1B等位基因平均产生803.1 g kg ^-1油酸。与仅具有一个突变FAD2-1-1等位基因的大豆品系相比，平均而言，其油酸平均高出约500 g kg ^-1。高油酸双突变体组的总油量更高（228.0 g kg ^-1）和蛋白质（401.0 g kg ^-1）与所有其他基因型组相比（P  <0.05）。总体而言，这种突变的FAD2-1A和FAD2-1B等位基因的特定组合似乎可以产生常规的高油酸大豆，而不会增加产量。