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Glutathione peroxidase 3 in the mud crab Scylla paramamosain: Characterization and regulation under nitrite stress.
Comparative Biochemistry and Physiology C: Toxicology & Pharmacology ( IF 3.9 ) Pub Date : 2019-11-21 , DOI: 10.1016/j.cbpc.2019.108673 Chang-Hong Cheng 1 , Hong-Ling Ma 1 , Yi-Qin Deng 1 , Juan Feng 1 , Xiao-Long Chen 1 , Zhi-Xun Guo 1
Comparative Biochemistry and Physiology C: Toxicology & Pharmacology ( IF 3.9 ) Pub Date : 2019-11-21 , DOI: 10.1016/j.cbpc.2019.108673 Chang-Hong Cheng 1 , Hong-Ling Ma 1 , Yi-Qin Deng 1 , Juan Feng 1 , Xiao-Long Chen 1 , Zhi-Xun Guo 1
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Glutathione peroxidases (GPx) are parts of the enzymatic antioxidant system that can eliminate the peroxides produced as effect of reactions of molecules with reactive oxygen species (ROS). In this study, a selenium-dependent glutathione peroxidase 3 cDNAs (designated as SpGPx3) was obtained from the mud crab Scylla paramamosain. The open reading frame (ORF) of SpGPx3 was 639 bp, which encoded a putative protein of 212 amino acids. SpGPx3 protein contained a characteristic GPx signature motif, and an active site motif. Mud crabs were exposed to 20 mg L-1 nitrite for 72 h. Quantitative real-time PCR analysis revealed that the SpGPx3 mRNA was distributed abundantly in mud crab. The transcript levels of antioxidant enzyme genes (SpGPx3, SpSOD and SpCAT) were obviously induced after acute nitrite exposure. After knockdown of the SpGPx3 level, the mortality of mud crabs and malondialdehyde (MDA) content significantly increased under nitrite stress. These results suggested that SpGPx3 played an important role in protecting organisms against oxidative stress.
中文翻译：
泥蟹Scylla paramamosain中的谷胱甘肽过氧化物酶3：亚硝酸盐胁迫下的特性和调控。
谷胱甘肽过氧化物酶（GPx）是酶促抗氧化剂系统的一部分，可以消除由于分子与活性氧（ROS）的反应而产生的过氧化物。在这项研究中，从泥蟹Scylla paramamosain获得了硒依赖性谷胱甘肽过氧化物酶3 cDNA（称为SpGPx3）。SpGPx3的开放阅读框（ORF）为639 bp，编码一个212个氨基酸的推定蛋白质。SpGPx3蛋白包含一个特征性的GPx签名基序和一个活动位点基序。将泥蟹暴露于20 mg L-1亚硝酸盐72小时。实时定量PCR分析表明，SpGPx3 mRNA在泥蟹中分布丰富。急性亚硝酸盐暴露后，明显诱导了抗氧化酶基因（SpGPx3，SpSOD和SpCAT）的转录水平。击倒SpGPx3级别后，亚硝酸盐胁迫下泥蟹的死亡率和丙二醛（MDA）含量明显增加。这些结果表明，SpGPx3在保护生物体免受氧化应激方面起着重要作用。
更新日期：2019-11-21
中文翻译：
泥蟹Scylla paramamosain中的谷胱甘肽过氧化物酶3：亚硝酸盐胁迫下的特性和调控。
谷胱甘肽过氧化物酶（GPx）是酶促抗氧化剂系统的一部分，可以消除由于分子与活性氧（ROS）的反应而产生的过氧化物。在这项研究中，从泥蟹Scylla paramamosain获得了硒依赖性谷胱甘肽过氧化物酶3 cDNA（称为SpGPx3）。SpGPx3的开放阅读框（ORF）为639 bp，编码一个212个氨基酸的推定蛋白质。SpGPx3蛋白包含一个特征性的GPx签名基序和一个活动位点基序。将泥蟹暴露于20 mg L-1亚硝酸盐72小时。实时定量PCR分析表明，SpGPx3 mRNA在泥蟹中分布丰富。急性亚硝酸盐暴露后，明显诱导了抗氧化酶基因（SpGPx3，SpSOD和SpCAT）的转录水平。击倒SpGPx3级别后，亚硝酸盐胁迫下泥蟹的死亡率和丙二醛（MDA）含量明显增加。这些结果表明，SpGPx3在保护生物体免受氧化应激方面起着重要作用。
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