Abstract C-phycocyanin (C-PC) is a phycobiliprotein that can be used as a natural blue dye in the food and cosmetic industries, as a biomarker or as an agent in medical treatments, depending on its purity grade. Here we described for the first time a single-step purification process of C-PC extracted from the wet biomass of Spirulina (Arthrospira) platensis LEB-52 using ion exchange chromatography with pH gradient elution. Different conditions varying the elution buffers and volumes, the loading pH and the addition of salt in the elution buffer were studied. The chromatographic condition that resulted in high recovery and purity consisted in equilibration and washing with 0.025 mol/L Tris-HCl buffer pH 6.5 and elution combining a step with 0.08 mol/L NaCl in 0.025 mol/L Tris-HCl buffer pH 6.5 and a pH gradient elution with 0.05 mol/L citrate buffer pH 6.2–3.0. This process resulted in C-PC with purities of 4.2 and 3.5 with recoveries of 32.6 and 49.5 %, respectively, in one purification step.

中文翻译：

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通过一步纯化过程获得的分析级 C-藻蓝蛋白

摘要 C-藻蓝蛋白（C-PC）是一种藻胆蛋白，根据其纯度等级，可在食品和化妆品工业中用作天然蓝色染料、生物标志物或药物治疗剂。在这里，我们首次描述了使用带 pH 梯度洗脱的离子交换色谱法从钝顶螺旋藻 (Arthrospira) LEB-52 的湿生物质中提取的 C-PC 的单步纯化过程。研究了改变洗脱缓冲液和体积、加载 pH 值和在洗脱缓冲液中添加盐的不同条件。导致高回收率和纯度的色谱条件包括平衡和洗涤，用 0.025 mol/L Tris-HCl 缓冲液 pH 6.5 和洗脱组合步骤与 0.08 mol/L NaCl 的 0.025 mol/L Tris-HCl 缓冲液 pH 6.5 和pH 梯度洗脱，0。05 mol/L 柠檬酸盐缓冲液 pH 6.2–3.0。这一过程在一个纯化步骤中产生了纯度为 4.2 和 3.5 的 C-PC，回收率分别为 32.6 和 49.5 %。