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Sandwich-type electrochemical immunosensor for carcinoembryonic antigen detection based on the cooperation of a gold-vertical graphene electrode and gold@silica-methylene blue.
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2019-11-21 , DOI: 10.1039/c9tb01803d Siyu Zheng 1 , Mingji Li 2 , Hongji Li 3 , Cuiping Li 1 , Penghai Li 1 , Lirong Qian 1 , Baohe Yang 1
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2019-11-21 , DOI: 10.1039/c9tb01803d Siyu Zheng 1 , Mingji Li 2 , Hongji Li 3 , Cuiping Li 1 , Penghai Li 1 , Lirong Qian 1 , Baohe Yang 1
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In this study, a sandwich-type electrochemical (EC) immunosensor was proposed to detect a carcinoembryonic antigen (CEA) based on Au–graphene and Au@SiO2–methylene blue (MB). The Au nanoparticles (NPs)–vertical graphene (VG) electrode efficiently amplifies the response signal by immobilizing a large amount of the coating antibody (Ab) and is characterized by excellent electrocatalytic activity. The MB nanodot-loaded Au@SiO2 carriers with core–shell nanostructure and detection Ab were used to construct the Ab–Au@SiO2–MB label, which improved the sensitivity due to the high EC signal of MB nanodots and the high labeling effect between the detection Ab and MB probe. A novel double-Ab sandwich strategy was developed to further improve the sensitivity and stability based on the same specificity of the coating and detection Abs for the recognition of CEA. Under optimal conditions, the developed EC sensor exhibited a wide linear range from 1 fg mL−1 to 100 ng mL−1, with an ultralow detection limit of 0.8 fg mL−1 (S/N = 3). The feasibility in the clinical application of the EC sensor was verified by the in vitro detection of CEA in human serum.
中文翻译：
基于金-垂直石墨烯电极和金@二氧化硅-亚甲基蓝的协同作用的夹心型电化学免疫传感器，用于癌胚抗原检测。
在这项研究中，提出了一种夹心型电化学（EC）免疫传感器，用于检测基于Au-石墨烯和Au @ SiO 2-亚甲基蓝（MB）的癌胚抗原（CEA ）。金纳米颗粒（NPs）-垂直石墨烯（VG）电极通过固定大量包被抗体（Ab）来有效放大响应信号，并具有出色的电催化活性。具有核壳纳米结构和检测抗体的MB纳米点负载的Au @ SiO 2载体被用于构建Ab–Au @ SiO 2-MB标记，由于MB纳米点的高EC信号以及检测Ab和MB探针之间的高标记效果，从而提高了灵敏度。基于涂层和检测抗体的相同特异性，开发了一种新颖的双抗体夹心策略，以进一步提高灵敏度和稳定性，以识别CEA。在最佳条件下，开发的EC传感器的线性范围从1 fg mL -1到100 ng mL -1，检测限为0.8 fg mL -1（S / N = 3）。通过体外检测人血清中的CEA验证了EC传感器在临床应用中的可行性。
更新日期：2019-11-21
中文翻译：
基于金-垂直石墨烯电极和金@二氧化硅-亚甲基蓝的协同作用的夹心型电化学免疫传感器，用于癌胚抗原检测。
在这项研究中，提出了一种夹心型电化学（EC）免疫传感器，用于检测基于Au-石墨烯和Au @ SiO 2-亚甲基蓝（MB）的癌胚抗原（CEA ）。金纳米颗粒（NPs）-垂直石墨烯（VG）电极通过固定大量包被抗体（Ab）来有效放大响应信号，并具有出色的电催化活性。具有核壳纳米结构和检测抗体的MB纳米点负载的Au @ SiO 2载体被用于构建Ab–Au @ SiO 2-MB标记，由于MB纳米点的高EC信号以及检测Ab和MB探针之间的高标记效果，从而提高了灵敏度。基于涂层和检测抗体的相同特异性，开发了一种新颖的双抗体夹心策略，以进一步提高灵敏度和稳定性，以识别CEA。在最佳条件下，开发的EC传感器的线性范围从1 fg mL -1到100 ng mL -1，检测限为0.8 fg mL -1（S / N = 3）。通过体外检测人血清中的CEA验证了EC传感器在临床应用中的可行性。
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