Common structural features facilitate the simultaneous identification and quantification of the five most common juvenile hormones by liquid chromatography-tandem mass spectrometry.,Insect Biochemistry and Molecular Biology

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Common structural features facilitate the simultaneous identification and quantification of the five most common juvenile hormones by liquid chromatography-tandem mass spectrometry.
Insect Biochemistry and Molecular Biology ( IF 3.2 ) Pub Date : 2019-11-21 , DOI: 10.1016/j.ibmb.2019.103287
Cesar E Ramirez ₁ , Marcela Nouzova ₂ , Veronika Michalkova ₃ , Francisco Fernandez-Lima ₄ , Fernando G Noriega ₅

Affiliation

This study reports the development and application of a liquid chromatography method coupled to electrospray tandem mass spectrometry (LC-MS/MS) for the identification and quantification of the five most common juvenile hormone (JH) homologs and methyl farnesoate (MF). The protocol allows the simultaneous analysis in a single LC run of JH I, JH II, JH III, JH III bisepoxide (JHB3) and JH III skipped bisepoxide (JHSB3). The identification of JHs is based on multiple reaction monitoring (MRM), using two of the most abundant fragmentation transitions for each hormone. Addition of deuterated JH III as an internal standard permits the absolute quantification of the different JHs. The JH homologs common structural features led to similar chromatographic behavior, as well as related fragmentation patterns, which facilitated the simultaneous detection of all the homologs in a single LC-MS/MS run. The protocol detects JHs in the low femtomole range, allowing often the analysis of JH in individual insects. Fragmentation of each of the JH homologs generates unique diagnostic ions that permitted the identification and quantification of JHs from samples of different species of Diptera, Lepidoptera, Heteroptera and Hymenoptera. Having a simple protocol, which can undisputedly determine the identity of the homologs present in a particular species, provides us with the opportunity to identify and quantify JHs existing in insects that are pests, vector of diseases or important research models.

中文翻译：

共同的结构特征有助于通过液相色谱-串联质谱法同时鉴定和定量五种最常见的少年激素。

这项研究报告了液相色谱法与电喷雾串联质谱联用（LC-MS / MS）的开发和应用，用于鉴定和定量五个最常见的少年激素（JH）同源物和法尼酸甲酯（MF）。该协议允许在单个LC运行中同时分析JH I，JH II，JH III，JH III双环氧化物（JHB3）和JH III跳过的双环氧化物（JHSB3）。JHs的鉴定基于多反应监测（MRM），其中每种激素使用两个最丰富的片段化跃迁。添加氘化的JH III作为内标可对不同的JH进行绝对定量。JH同源物的共同结构特征导致相似的色谱行为以及相关的碎片图谱，有助于在一次LC-MS / MS运行中同时检测所有同源物。该协议可检测到低飞虱范围内的JH，因此经常可以分析单个昆虫中的JH。每个JH同源物的片段化产生独特的诊断离子，从而可以从双翅目，鳞翅目，异翅目和膜翅目的不同物种的样品中鉴定和定量JHs。有了一个简单的协议，可以毫无疑问地确定特定物种中存在的同系物的身份，这为我们提供了机会来鉴定和量化存在于害虫，疾病媒介或重要研究模型的昆虫中的JH。每个JH同源物的片段化产生独特的诊断离子，从而可以从双翅目，鳞翅目，异翅目和膜翅目的不同物种的样品中鉴定和定量JHs。有了一个简单的协议，可以毫无疑问地确定特定物种中存在的同系物的身份，这为我们提供了机会来鉴定和量化存在于害虫，疾病媒介或重要研究模型的昆虫中的JH。每个JH同源物的片段化产生独特的诊断离子，从而可以从双翅目，鳞翅目，异翅目和膜翅目的不同物种的样品中鉴定和定量JHs。有了一个简单的协议，可以毫无疑问地确定特定物种中存在的同系物的身份，这为我们提供了机会来鉴定和量化存在于害虫，疾病媒介或重要研究模型的昆虫中的JH。

更新日期：2019-11-21

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