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Functional analysis of the three major PGRPLC isoforms in the midgut of the malaria mosquito Anopheles coluzzii.
Insect Biochemistry and Molecular Biology ( IF 3.2 ) Pub Date : 2019-11-21 , DOI: 10.1016/j.ibmb.2019.103288 Faye H Rodgers 1 , Julia A Cai 1 , Andre N Pitaluga 1 , Dominique Mengin-Lecreulx 2 , Mathilde Gendrin 3 , George K Christophides 1
Insect Biochemistry and Molecular Biology ( IF 3.2 ) Pub Date : 2019-11-21 , DOI: 10.1016/j.ibmb.2019.103288 Faye H Rodgers 1 , Julia A Cai 1 , Andre N Pitaluga 1 , Dominique Mengin-Lecreulx 2 , Mathilde Gendrin 3 , George K Christophides 1
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Peptidoglycan recognition proteins (PGRPs) constitute the primary means of bacterial recognition in insects. Recent work in the model organism Drosophila has revealed the mechanisms by which the complement of PGRPs refine the sensitivity of different tissues to bacterial elicitors, permitting the persistence of commensal bacteria in the gut whilst maintaining vigilance against bacterial infection. Here, we use in vivo knockdowns and in vitro pull-down assays to investigate the role of the three major isoforms of the transmembrane receptor of the Imd pathway, PGRPLC, in basal immunity in the Anopheles coluzzii mosquito midgut. Our results indicate that the mosquito midgut is regionalized in its expression of immune effectors and of PGRPLC1. We show that PGRPLC1 and PGRPLC3 are pulled down with polymeric DAP-type peptidoglycan, while PGRPLC2 and PGRPLC3 co-precipitate in the presence of TCT, a peptidoglycan monomer. These data suggest that, as found in Drosophila, discrimination of polymeric and monomeric PGN by Anopheles PGRPLC participates in the regulation of the Imd pathway.
中文翻译：
疟疾蚊子中肠中肠的三种主要PGRPLC同工型的功能分析。
肽聚糖识别蛋白（PGRP）构成昆虫中细菌识别的主要手段。模式生物果蝇的最新研究揭示了PGRP的互补作用可改善不同组织对细菌引发剂的敏感性的机制，从而使肠道内的共生细菌得以持久存在，同时保持对细菌感染的警惕性。在这里，我们使用体内击倒和体外下拉试验来研究Imd途径的跨膜受体PGRPLC的三种主要同工型在库蚊按蚊中肠基础免疫中的作用。我们的结果表明，蚊子中肠在免疫效应子和PGRPLC1的表达上是区域性的。我们显示PGRPLC1和PGRPLC3与聚合DAP型肽聚糖一起被下拉，而PGRPLC2和PGRPLC3在肽聚糖单体TCT的存在下共沉淀。这些数据表明，如在果蝇中发现的那样，按蚊PGRPLC对聚合物和单体PGN的区分参与了Imd途径的调节。
更新日期：2019-11-21
中文翻译：
疟疾蚊子中肠中肠的三种主要PGRPLC同工型的功能分析。
肽聚糖识别蛋白（PGRP）构成昆虫中细菌识别的主要手段。模式生物果蝇的最新研究揭示了PGRP的互补作用可改善不同组织对细菌引发剂的敏感性的机制，从而使肠道内的共生细菌得以持久存在，同时保持对细菌感染的警惕性。在这里，我们使用体内击倒和体外下拉试验来研究Imd途径的跨膜受体PGRPLC的三种主要同工型在库蚊按蚊中肠基础免疫中的作用。我们的结果表明，蚊子中肠在免疫效应子和PGRPLC1的表达上是区域性的。我们显示PGRPLC1和PGRPLC3与聚合DAP型肽聚糖一起被下拉，而PGRPLC2和PGRPLC3在肽聚糖单体TCT的存在下共沉淀。这些数据表明，如在果蝇中发现的那样，按蚊PGRPLC对聚合物和单体PGN的区分参与了Imd途径的调节。
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