RATIONALE Herbal dietary supplements (HDSs) adulterated with undeclared synthetic drugs can lead to serious health problems METHODS: A fast-switching positive/negative high-voltage (+/- HV) was developed to apply on electrospray ionization mass spectrometry (ESI-MS) with porous tips for rapid screening of five antirheumatic drugs in antirheumatic HDSs. The fast-switching (switch-time: 100 ms) negative and positive ions were alternately generated to perform full-MS and tandem-MS analysis, providing an effective method for rapid detection of analytes in whichever mode of detection was most suitable (negative or positive ion mode). The use of different tips and solvents was also optimized in this work. RESULTS The limits of detection of the five antirheumatic drugs were found to be less than 0.1 ng/g (S/N > 3). The reproducibility of the five drugs was measured to be 10.0-23.3% (n = 5). A single sample analysis could be completed within 1 min. Rapid screening of a total of 28 real HDS samples collected from the market was examined by the fast-switching HV substrate-tip ESI-MS method, and the screening result was further validated by conventional liquid chromatography/mass spectrometry. CONCLUSIONS Overall, our results demonstrated that fast-switching HV substrate-tip ESI-MS is a rapid, reliable, and effective method for simultaneous screening of various analytes in complex samples.

中文翻译：

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快速开关高压多孔尖端电喷雾电离质谱法，用于快速检测掺假草药膳食补充剂中的抗风湿药。

理由：掺有未申报合成药物的草药膳食补充剂（HDS）可能导致严重的健康问题。方法：开发了一种可快速切换的正/负高压（+/- HV），用于电喷雾电离质谱（ESI-MS）带有多孔尖端，可快速筛查抗风湿性HDS中的五种抗风湿药。交替产生快速切换（切换时间：100毫秒）的负离子和正离子，以执行全质谱和串联质谱分析，从而在最合适的检测方式（阴性或阴性）下提供了一种快速检测分析物的有效方法。正离子模式）。在这项工作中，还优化了不同技巧和溶剂的使用。结果发现五种抗风湿药的检出限小于0.1 ng / g（S / N> 3）。测得这五种药物的重现性为10.0-23.3％（n = 5）。一次样品分析可以在1分钟内完成。通过快速切换的HV底物尖端ESI-MS方法对从市场上收集的总共28个真实HDS样品进行了快速筛选，并通过常规液相色谱/质谱法进一步验证了筛选结果。结论总体而言，我们的结果表明，快速切换的HV底物尖端ESI-MS是一种快速，可靠且有效的方法，可同时筛查复杂样品中的各种分析物。通过常规液相色谱/质谱法进一步验证了筛选结果。结论总体而言，我们的结果表明，快速切换的HV底物尖端ESI-MS是一种快速，可靠且有效的方法，可同时筛查复杂样品中的各种分析物。通过常规液相色谱/质谱法进一步验证了筛选结果。结论总体而言，我们的结果表明，快速切换的HV底物尖端ESI-MS是一种快速，可靠且有效的方法，可同时筛查复杂样品中的各种分析物。