AIM Intraoperative electron Radiotherapy, herein referred to, as IOeRT is a novel approach in breast cancer (BC) treatment. This study designed to investigate short-term molecular effects of 12Gy as Boost versus 21Gy as Radical dose of IOeRT using high throughput approaches. MATERIALS AND METHODS Six BC patients as a pilot study were treated with IOeRT following two separate strategies, including Boost and Radical doses. Approximately 100 mg of tumor bed tissue retrieved from each patient (before IOeRT,immediately, 24 h post-treatment). mRNA sequencing also Isobaric tag for relative and absolute quantitation (iTRAQ) were performed to study the transcriptome and proteome profile of IOeRT-treated tumor bed. RESULTS Using NGS, ~6 Giga base (GB) clean data per individual samples were generated. Moreover, by iTRAQ for proteome quantification, in total, 1,045,410 spectrums were generated, likewise 5860 proteins were identified (FDR <0.01). CONCLUSION Functional annotation and gene ontology (GO) indicated that significant enrichment in molecular pathways on BC treatment is somehow single high dose-independent. This means that, key molecular pathways in radiotherapy (RT) are equally enriched by both Boost and Radical doses. Generally, by modification of the Radical dose, with the same effectiveness, it is possible to reduce single high dose irradiation in BC.

中文翻译：

---

转录组学和蛋白质组学方法揭示了乳腺癌患者术中放射治疗后肿瘤床修饰的生物学基础：一项先导研究。

AIM术中电子放射疗法，在本文中称为IOeRT，是乳腺癌（BC）治疗中的一种新颖方法。这项研究旨在使用高通量方法研究作为IOeRT自由基剂量的12Gy作为Boost的短期分子效应和21Gy作为IOeRT的自由基剂量的短期分子效应。材料与方法作为一项初步研究的6例BC患者接受IOeRT治疗，遵循两种单独的策略，包括加强剂量和自由基剂量。从每个患者中回收大约100 mg的肿瘤床组织（在IOeRT之前，即治疗后24小时立即）。进行了mRNA测序以及相对定量和绝对定量等压标记（iTRAQ），以研究IOeRT处理的肿瘤床的转录组和蛋白质组图谱。结果使用NGS，每个单独的样品产生了约6 Giga base（GB）干净数据。此外，通过iTRAQ进行蛋白质组定量，总共产生1,045,410个光谱，同样鉴定出5860个蛋白质（FDR <0.01）。结论功能注释和基因本体论（GO）表明，在BC治疗中分子途径的显着富集以某种方式独立于高剂量。这意味着，Boost和Radical剂量均会同样丰富放射疗法（RT）中的关键分子途径。通常，通过以相同的效力改变自由基剂量，可以减少BC中的单次高剂量照射。Boost和Radical剂量同样丰富了放射治疗（RT）中的关键分子途径。通常，通过以相同的效力改变自由基剂量，可以减少BC中的单次高剂量照射。Boost和Radical剂量同样丰富了放射治疗（RT）中的关键分子途径。通常，通过以相同的效力改变自由基剂量，可以减少BC中的单次高剂量照射。