Abstract In this study, we are successfully fabricated on a hydrogel consisting of TiO2 nanoparticles loaded onto a gelatin/chitosan matrix to control the acceleration of bone fracture healing and improved the nursing care applications. Each specimen (chitosan, gelatin and titanium dioxide) were characterized and confirmed by using different techniques, Fourier transforms infrared spectroscopy, X-ray diffraction analysis, Scanning Electron Microscopy with Elemental dispersive X-ray analysis, Thermo-gravimetric and Differential thermal analysis. In addition, the cell cytotoxicity results verified that the TiO2/gelatin-chitosan hydrogel are nontoxic to osteoblasts. And cell fixation outcome after 5 days of incubation condition revels that the enhanced in vitro cell survival and cell spreading on the prepared TiO2 incorporated hydrogel with respect to gelatin/chitosan hydrogel. Furthermore, TiO2/gelatin-chitosan hydrogel nanostructures can modulate the bone fracture healing, indicating a potential application on nursing care.

中文翻译：

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明胶/壳聚糖共聚生物复合水凝胶系统新型形态定制纳米结构的制备，以加速骨折愈合和硬组织护理管理

摘要 在这项研究中，我们成功地制备了由负载在明胶/壳聚糖基质上的 TiO2 纳米颗粒组成的水凝胶，以控制骨折愈合的加速并改善护理应用。每个样品（壳聚糖、明胶和二氧化钛）通过使用不同的技术、傅立叶变换红外光谱、X 射线衍射分析、具有元素色散 X 射线分析的扫描电子显微镜、热重分析和差热分析进行表征和确认。此外，细胞毒性结果证实，TiO2/明胶-壳聚糖水凝胶对成骨细胞无毒。培养 5 天后的细胞固定结果表明，相对于明胶/壳聚糖水凝胶，在制备的 TiO2 掺入水凝胶上增强的体外细胞存活和细胞扩散。此外，TiO2/明胶-壳聚糖水凝胶纳米结构可以调节骨折愈合，表明其在护理方面的潜在应用。