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Optimization, kinetics, and radioprotective potential of a heteropolysaccharide from L. rhamnosus RVPI
Biotechnology and Applied Biochemistry ( IF 3.2 ) Pub Date : 2019-11-20 , DOI: 10.1002/bab.1855 Anushri Umesh 1 , Pradeepa 1 , Anushree Manjunath 1 , Chithralekha Salian 1 , Manasadeepika 1 , Manu Suvarna 2 , Vidya Shimoga Muddappa 1
Biotechnology and Applied Biochemistry ( IF 3.2 ) Pub Date : 2019-11-20 , DOI: 10.1002/bab.1855 Anushri Umesh 1 , Pradeepa 1 , Anushree Manjunath 1 , Chithralekha Salian 1 , Manasadeepika 1 , Manu Suvarna 2 , Vidya Shimoga Muddappa 1
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The probiotic potential of Lactobacillus rhamnosus RVP1 isolated from Sardinella longiceps was investigated in vitro . The bacterium exhibited highest tolerance at low pH and high bile salt concentration and demonstrated good antioxidant activity, hydrophobicity, and inhibited both Gram‐negative and Gram‐positive indicator bacteria. To aid in process design and to unravel the fermentation kinetics, response surface methodology was devised to optimize the EPS production from L. rhamnosus and mechanistic models were developed to describe the fermentation kinetics. The optimum pH and dextrose and peptone concentrations for EPS production were 7.07 and 19.995 and 23.4 g/L, respectively, with a predicted yield of 724 mg/L. The actual yield under these conditions was 708 ± 29 mg/L that was within the 95% confidence interval. The simulated mechanistic model fit the experimental values with a high degree of correlation with R 2 = 0.99, 0.96, and 0.97 for the logistic growth, substrate consumption, and EPS production and degradation curves, respectively. The kinetic constants µmax = 0.29/H, X max = 3.44 g/L, k f = 348 mg of EPS/g of dry biomass and k d = 0.53/H were derived from the model. The EPS administration improved the survival of irradiated mice by 50%, proving its radioprotective potential and showed positive effects on structural integrity of intestinal tissue.
中文翻译：
鼠李糖乳杆菌 RVPI 杂多糖的优化、动力学和辐射防护潜力
体外研究了从长头沙丁鱼中分离出的鼠李糖乳杆菌RVP1 的益生潜力。该细菌在低pH值和高胆盐浓度下表现出最高的耐受性，并表现出良好的抗氧化活性和疏水性，并能抑制革兰氏阴性和革兰氏阳性指示菌。为了帮助工艺设计和阐明发酵动力学，设计了响应面方法来优化鼠李糖乳杆菌的 EPS 生产，并开发了机械模型来描述发酵动力学。 EPS 生产的最佳 pH 值、葡萄糖和蛋白胨浓度分别为 7.07、19.995 和 23.4 g/L，预计产量为 724 mg/L。这些条件下的实际产量为 708 ± 29 mg/L，在 95% 置信区间内。模拟机械模型与实验值拟合，物流增长、基质消耗、EPS 生产和降解曲线分别与R 2 = 0.99、0.96 和 0.97 高度相关。从模型中得出动力学常数 µ max = 0.29/H、 X max = 3.44 g/L、 k f = 348 mg EPS/g 干生物质和k d = 0.53/H。 EPS 给药使受辐射小鼠的存活率提高了 50%，证明了其辐射防护潜力，并对肠道组织的结构完整性产生了积极影响。
更新日期：2019-11-20
中文翻译：
鼠李糖乳杆菌 RVPI 杂多糖的优化、动力学和辐射防护潜力
体外研究了从长头沙丁鱼中分离出的鼠李糖乳杆菌RVP1 的益生潜力。该细菌在低pH值和高胆盐浓度下表现出最高的耐受性，并表现出良好的抗氧化活性和疏水性，并能抑制革兰氏阴性和革兰氏阳性指示菌。为了帮助工艺设计和阐明发酵动力学，设计了响应面方法来优化鼠李糖乳杆菌的 EPS 生产，并开发了机械模型来描述发酵动力学。 EPS 生产的最佳 pH 值、葡萄糖和蛋白胨浓度分别为 7.07、19.995 和 23.4 g/L，预计产量为 724 mg/L。这些条件下的实际产量为 708 ± 29 mg/L，在 95% 置信区间内。模拟机械模型与实验值拟合，物流增长、基质消耗、EPS 生产和降解曲线分别与R 2 = 0.99、0.96 和 0.97 高度相关。从模型中得出动力学常数 µ max = 0.29/H、 X max = 3.44 g/L、 k f = 348 mg EPS/g 干生物质和k d = 0.53/H。 EPS 给药使受辐射小鼠的存活率提高了 50%，证明了其辐射防护潜力，并对肠道组织的结构完整性产生了积极影响。
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