Propofol and cisatracurium besylate have been simultaneously determined using a highly sensitive first derivative synchronous spectrofluorometric method. The method is based on measuring first derivative synchronous spectrofluorimetric amplitude at Δλ = 40 nm with a scanning rate of 600 nm/min. The different experimental parameters affecting the fluorescence intensity of the two drugs were carefully studied and optimized. The amplitude-concentration plots were rectilinear over the range 40.0-400.0 ng/mL and 20.0-280.0 ng/mL for propofol and cisatracurium, respectively with lower detection limits of 4.0 and 2.35 ng/mL and quantification limits of 12.1 and 7.1 ng/mL for propofol and cisatracurium, respectively. The proposed method was successfully applied for the determination of the two compounds in synthetic mixtures and in commercial ampoules. The high sensitivity attained using the proposed method allowed the simultaneous determination of both drugs in spiked plasma samples. The mean % recoveries in spiked human plasma (n = 3) were 96.53 ± 0.90 and 96.20 ± 1.64 for each of propofol and cisatracurium, respectively. The method was validated in compliance with International Council of Harmonization (ICH) Guidelines.

中文翻译：

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一阶导数同步荧光光谱法同时测定生物液体中的异丙酚和苯磺酸顺式曲库铵。

使用高灵敏度的一阶导数同步荧光光谱法同时测定了异丙酚和苯磺酸顺式曲库铵。该方法基于以600 nm / min的扫描速度测量Δλ= 40 nm时的一阶导数同步光谱荧光幅度。仔细研究和优化了影响两种药物荧光强度的不同实验参数。异丙酚和顺式曲库铵的幅度-浓度图在40.0-400.0 ng / mL和20.0-280.0 ng / mL范围内呈直线变化，检出限较低，分别为4.0和2.35 ng / mL，定量限为12.1和7.1 ng / mL分别用于异丙酚和西沙曲库铵。所提出的方法已成功地用于测定合成混合物和市售安瓿中的两种化合物。使用建议的方法获得的高灵敏度允许同时测定加标血浆样品中的两种药物。加标人血浆（n = 3）中丙泊酚和西沙曲库铵的平均回收率分别为96.53±0.90和96.20±1.64。该方法已根据国际协调理事会（ICH）指南进行了验证。