An Inorganic Biopolymer Polyphosphate Controls Positively Charged Protein Phase Transitions.,Angewandte Chemie International Edition

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An Inorganic Biopolymer Polyphosphate Controls Positively Charged Protein Phase Transitions.
Angewandte Chemie International Edition ( IF 16.1 ) Pub Date : 2019-11-19 , DOI: 10.1002/anie.201913833
Xin Wang _{1,

2} , Chenke Shi ₂ , Jianbin Mo ₁ , Yun Xu ₂ , Wei Wei _{1,

2,

3} , Jing Zhao _{1,

3}

Affiliation

Polyphosphate (PolyP) is one of the most compact inorganic polyanionic biopolymers that participates in various physiological processes. However, the mechanism of the interaction between polyP and proteins remains poorly understood. Herein, we report that polyP can interact with positively charged green fluorescent protein, +36GFP, resulting in liquid-liquid phase separation (LLPS) by intermolecular electrostatic interactions in cells. Upon nutrient deprivation, genetically engineered Citrobacter freundii accumulates intracellular polyP at a rate of 210 μm min-1 , resulting in the compartmentation of +36GFP at the cell poles within 1 h. Medium chain-length polyP (60-mer) could induce the formation of +36GFP coacervates in vitro at a protein concentration as low as 200 nm, which is of the same magnitude as native proteins. In contrast, shorter polyP (14-mer) could not induce LLPS under the same conditions. This may offer a general approach to manipulate protein-protein interactions through LLPS.

中文翻译：

无机生物聚合物多磷酸盐控制带正电的蛋白质相变。

聚磷酸盐（PolyP）是参与各种生理过程的最紧凑的无机聚阴离子生物聚合物之一。但是，polyP和蛋白质之间相互作用的机制仍然知之甚少。在这里，我们报道polyP可以与带正电的绿色荧光蛋白+ 36GFP相互作用，通过细胞内的分子间静电相互作用导致液-液相分离（LLPS）。营养物质剥夺后，基因改造的弗氏柠檬酸杆菌以210μmmin-1的速率积累细胞内polyP，导致+ 36GFP在1 h内在细胞极处分隔。中链长的polyP（60-mer）可以在低至200 nm的蛋白质浓度下在体外诱导+ 36GFP凝聚层的形成，其大小与天然蛋白质相同。相比之下，较短的polyP（14-mer）在相同条件下不能诱导LLPS。这可能提供通过LLPS操纵蛋白质间相互作用的一般方法。

更新日期：2020-01-16

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