ABSTRACT As an effective alternative to traditional pesticides, ozonated water has been used for direct immersion and spraying applications, respectively, against the pathogenic bacterium Ralstonia solanacearum (R. solanacearum) and fungus Alternaria solani (A. solani) that are pathogens of tomato. Immersing R. solanacearum in 0.8 mg/L ozonated water resulted in complete inactivation, while the growth-inhibition rates of the A. solani radial mycelia gradually increased with a 67.0% decrease compared to those of the control at an ozonated water concentration of 7.5 mg/L. This indicated that ozonated water was effective at restraining pathogens in the tomato plant. In addition, spraying wild tomato species (Solanum lycopersicum) plants with ozonated water at concentrations below 10 mg/L for 25 d had no negative effects after treatment compared to those of the control. Photosynthetic activities and the antioxidant system response in the untreated and ozonated water-treated plant leaves had no observable changes. To determine the optimal ozonated water concentration for the pathogen and its host plant, we examined a variety of modes of action, and then, below 10 mg/L of ozonated water. Thus, ozonated water not only inhibits the proliferation of plant pathogens, but is an effective alternative to the pesticides traditionally used to prevent disease in agricultural production.

中文翻译：

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不同浓度臭氧对体外植物病原体发育、番茄产量和品质、光合活性和酶活性的影响

摘要 作为传统杀虫剂的有效替代品，臭氧水已分别用于直接浸泡和喷洒应用，分别用于对抗病原菌青枯病菌 (R. solanacearum) 和真菌黑链孢菌 (A. solani)，它们是番茄的病原体。将青枯菌浸泡在 0.8 mg/L 的臭氧水中导致完全灭活，而在臭氧水浓度为 7.5 mg 时，青枯菌径向菌丝体的生长抑制率逐渐增加，与对照相比下降了 67.0% /L。这表明臭氧水可有效抑制番茄植株中的病原体。此外，与对照相比，用浓度低于 10 mg/L 的臭氧水喷洒野生番茄 (Solanum lycopersicum) 植物 25 天后，处理后没有负面影响。未经处理和经臭氧水处理的植物叶片的光合活性和抗氧化系统反应没有可观察到的变化。为了确定病原体及其寄主植物的最佳臭氧水浓度，我们检查了多种作用模式，然后是低于 10 毫克/升的臭氧水。因此，臭氧水不仅可以抑制植物病原体的增殖，而且是传统上用于预防农业生产疾病的农药的有效替代品。未经处理和经臭氧水处理的植物叶片的光合活性和抗氧化系统反应没有可观察到的变化。为了确定病原体及其寄主植物的最佳臭氧水浓度，我们检查了多种作用模式，然后是低于 10 毫克/升的臭氧水。因此，臭氧水不仅可以抑制植物病原体的增殖，而且是传统上用于预防农业生产疾病的农药的有效替代品。未经处理和经臭氧水处理的植物叶片的光合活性和抗氧化系统反应没有可观察到的变化。为了确定病原体及其寄主植物的最佳臭氧水浓度，我们检查了多种作用模式，然后是低于 10 毫克/升的臭氧水。因此，臭氧水不仅可以抑制植物病原体的增殖，而且是传统上用于预防农业生产疾病的农药的有效替代品。