当前位置: X-MOL 学术Blood Cancer J. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Transcriptome analysis reveals significant differences between primary plasma cell leukemia and multiple myeloma even when sharing a similar genetic background.
Blood Cancer Journal ( IF 12.9 ) Pub Date : 2019-11-20 , DOI: 10.1038/s41408-019-0253-1
Elizabeta A Rojas 1, 2 , Luis A Corchete 1, 2 , María Victoria Mateos 1, 2, 3 , Ramón García-Sanz 1, 2, 3, 4 , Irena Misiewicz-Krzeminska 1, 2, 5 , Norma C Gutiérrez 1, 2, 3, 4
Affiliation  

Primary plasma cell leukemia (pPCL) is a highly aggressive plasma cell dyscrasia characterised by short remissions and very poor survival. Although the 17p deletion is associated with poor outcome and extramedullary disease in MM, its presence does not confer the degree of aggressiveness observed in pPCL. The comprehensive exploration of isoform expression and RNA splicing events may provide novel information about biological differences between the two diseases. Transcriptomic studies were carried out in nine newly diagnosed pPCL and ten MM samples, all of which harbored the 17p deletion. Unsupervised cluster analysis clearly distinguished pPCL from MM samples. In total 3584 genes and 20033 isoforms were found to be deregulated between pPCL and MM. There were 2727 significantly deregulated isoforms of non-differentially expressed genes. Strangely enough, significant differences were observed in the expression of spliceosomal machinery components between pPCL and MM, in respect of the gene, isoform and the alternative splicing events expression. In summary, transcriptome analysis revealed significant differences in the relative abundance of isoforms between pPCL and MM, even when they both had the 17p deletion. The mRNA processing pathway including RNA splicing machinery emerged as one of the most remarkable mechanisms underlying the biological differences between the two entities.

中文翻译:

转录组分析显示,即使共享相似的遗传背景,原发性浆细胞白血病与多发性骨髓瘤之间也存在显着差异。

原发性浆细胞白血病(pPCL)是一种高度侵袭性的浆细胞发育不良,其特征在于缓解期短且生存期很差。尽管17p缺失与MM的不良预后和髓外疾病有关,但它的存在并不赋予pPCL所观察到的侵略性。对同工型表达和RNA剪接事件的全面探索可能会提供有关两种疾病之间的生物学差异的新信息。在9个新诊断的pPCL和10个MM样品中进行了转录组学研究,所有这些样品均含有17p缺失。无监督聚类分析清楚地将pPCL与MM样品区分开。总共发现pPCL和MM之间的3584个基因和20033个同工型被解除调节。有2727个显着失调的非差异表达基因亚型。足够奇怪的是,在基因,同工型和替代剪接事件的表达方面,在pPCL和MM之间的剪接体机械成分的表达中观察到了显着差异。总之,转录组分析显示pPCL和MM之间同工型的相对丰度存在显着差异,即使它们都具有17p缺失也是如此。包括RNA剪接机制在内的mRNA加工途径已成为两个实体之间生物学差异的最显着机制之一。转录组分析显示pPCL和MM之间同工型的相对丰度存在显着差异,即使它们都具有17p缺失也是如此。包括RNA剪接机制在内的mRNA加工途径已成为两个实体之间生物学差异的最显着机制之一。转录组分析显示pPCL和MM之间同工型的相对丰度存在显着差异,即使它们都具有17p缺失也是如此。包括RNA剪接机制在内的mRNA加工途径已成为两个实体之间生物学差异的最显着机制之一。
更新日期:2019-11-20
down
wechat
bug