During the course of screening for new metabolites from basidiomycetes, we isolated and characterized five previously undescribed secondary metabolites, skeletocutins M–Q (1–5), along with the known metabolite tyromycin A (6) from the fruiting bodies of the polypore Skeletocutis sp. The new compounds did not exhibit any antimicrobial, cytotoxic, or nematicidal activities. However, compound 3 moderately inhibited the biofilm formation of Staphylococcus aureus (S. aureus), while compounds 3 and 4 performed moderately in the ʟ-leucine-7-amido-4-methylcoumarin (ʟ-Leu-AMC) inhibition assay. These compounds represent the first secondary metabolites reported to occur in the fruiting bodies by Skeletocutis. Interestingly, tyromycin A (6) was found to be the only common metabolite in fruiting bodies and mycelial cultures of the fungus, and none of the recently reported skeletocutins from the culture of the same strain were detected in the basidiomes.

中文翻译：

---

Skeletocutins M–Q：担子菌Skeletocutis sp。子实体的生物活性化合物。在非洲收集

在筛选从担子菌新代谢物的过程中，我们分离和表征5以前未描述的次级代谢产物，skeletocutins M-Q（1 - 5），与已知的代谢物tyromycin A（沿6从多孔菌的子实体）Skeletocutis属。新化合物没有表现出任何抗微生物，细胞毒性或杀线虫活性。但是，化合物3适度抑制金黄色葡萄球菌（S. aureus）的生物膜形成，而化合物3和4在β-亮氨酸-7-酰胺基-4-甲基香豆素（β-Leu-AMC）抑制试验中适度进行。这些化合物代表Skeletocutis报道的在子实体中发生的第一个次生代谢物。有趣的是，酪氨酸A（6）被发现是真菌子实体和菌丝体培养物中唯一的常见代谢产物，最近在该菌株中未检测到同一菌株培养物中的骨架角质素。