Polyphenols are a group of chemical substances found in plants, with immunomodulatory, antiproliferative, and anti-inflammatory properties that might be useful in the prophylaxis and treatment of graft-versus-host disease (GVHD). Polyphenolic extract (PE) obtained from extra virgin olive oil (EVOO) decreased the activation and proliferation of activated T cells. In addition, a decreased production of proinflammatory cytokines was observed upon exposure to PE. Western blot assays showed a marked inhibition of Akt phosphorylation and nuclear translocation of NF-κB in activated T cells. In a murine model of acute GVHD, we observed that mice that received a diet supplemented in PE (600 ppm) presented a higher survival rate and lower risk of developing GVHD when compared with the group that received a control diet. Histopathologic examination showed a significantly lower gut involvement in mice receiving PE, with a decrease in proinflammatory cytokines (IL-2, IL-17, and TNF-α) in serum and the reestablishment of butyrate concentration in the gut. In conclusion, PE obtained from EVOO exerted a potent immunomodulatory effect, reducing the activation and proliferation of activated T cells and the production of proinflammatory cytokines. In a murine model of acute GVHD, a PE-supplemented diet reduced the incidence and severity of the disease and increased survival after transplantation.

中文翻译：

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橄榄油中的多酚提取物（PE）具有强大的免疫调节作用，可在小鼠模型中预防移植物抗宿主病。

多酚是植物中发现的一组化学物质，具有免疫调节，抗增殖和抗炎特性，可能有助于预防和治疗移植物抗宿主病（GVHD）。从特级初榨橄榄油（EVOO）获得的多酚提取物（PE）降低了活化T细胞的活化和增殖。另外，在暴露于PE时，观察到促炎细胞因子的产生减少。蛋白质印迹分析显示，在活化的T细胞中，Akt磷酸化和NF-κB的核易位明显受到抑制。在急性GVHD的小鼠模型中，我们观察到，与接受对照饮食的组相比，接受PE补充饮食（600 ppm）的小鼠表现出更高的存活率和更低的发生GVHD的风险。组织病理学检查显示，接受PE的小鼠的肠脏受累明显减少，血清中的促炎细胞因子（IL-2，IL-17和TNF-α）降低，肠内丁酸盐浓度重新建立。总之，从EVOO获得的PE发挥有效的免疫调节作用，减少了活化的T细胞的活化和增殖以及促炎细胞因子的产生。在急性GVHD的小鼠模型中，补充PE的饮食降低了疾病的发生率和严重性，并提高了移植后的生存率。减少活化的T细胞的活化和增殖以及促炎性细胞因子的产生。在急性GVHD的小鼠模型中，补充PE的饮食降低了疾病的发生率和严重性，并提高了移植后的生存率。减少活化的T细胞的活化和增殖以及促炎性细胞因子的产生。在急性GVHD的小鼠模型中，补充PE的饮食降低了疾病的发生率和严重性，并提高了移植后的生存率。