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The role of phospholipid composition and ergosterol presence in the adaptation of fungal membranes to harsh environmental conditions-membrane modeling study.
Biochimica et Biophysica Acta (BBA) - Biomembranes ( IF 2.8 ) Pub Date : 2019-11-18 , DOI: 10.1016/j.bbamem.2019.183136 Paulina Perczyk 1 , Aneta Wójcik 1 , Paweł Wydro 2 , Marcin Broniatowski 1
Biochimica et Biophysica Acta (BBA) - Biomembranes ( IF 2.8 ) Pub Date : 2019-11-18 , DOI: 10.1016/j.bbamem.2019.183136 Paulina Perczyk 1 , Aneta Wójcik 1 , Paweł Wydro 2 , Marcin Broniatowski 1
Affiliation
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Soil fungi play an important role in the environment decomposing dead organic matter and degrading persistent organic pollutants (POP). The presence of hydrophobic POP in the soil and membrane-lytic substances excreted by competing microorganism to the soil solution is the constant threat to these organisms. To survive in the harsh environment and counteract these hazards the fungal cells have to strictly control the composition of the lipids in their cellular membranes. However, in the case of fungal membranes the correlation between their composition and physical properties is not fully understood. In our studies we applied Langmuir monolayers formed by phospholipids typical to fungal membranes and ergosterol as versatile model membranes. These membranes were characterized by the Langmuir technique, Brewster Angle Microscopy and Grazing Incidence X-ray Diffraction, as well as were exposed to the action of phospholipase A2 treated as a model membrane-lytic protein. We started our studies from the equimolar mixture of phosphatidylethanolamine with phosphatidylcholine and doped this matrix with phosphatidylserine (PS) or phosphatidylinositol (PI). It turned out that the membranes with PS were much more condensed at the mesoscale and periodically organized at the molecular level. Starting from these models we derived two families of model fungal membranes adding to these phospholipid matrices ergosterol. It turned out that the level of ergosterol content is of crucial importance for the model membrane structure and its durability. Changing the ergosterol mole ratio from 0 to 0.5 we defined and described in detail four different 2D crystalline phases.
中文翻译：
磷脂成分和麦角甾醇的存在在真菌膜适应恶劣环境条件中的作用-膜建模研究。
土壤真菌在分解死有机物和降解持久性有机污染物（POP）的环境中起着重要作用。土壤中疏水性POP的存在以及被竞争性微生物排泄到土壤溶液中的膜溶解性物质不断威胁着这些生物。为了在恶劣的环境中生存并抵消这些危害，真菌细胞必须严格控制其细胞膜中脂质的组成。然而，在真菌膜的情况下，其组成和物理性质之间的相关性还没有被完全理解。在我们的研究中，我们应用了由真菌膜和麦角固醇典型的磷脂形成的Langmuir单层膜作为通用模型膜。这些膜通过Langmuir技术进行了表征，布鲁斯特角显微镜和掠入射X射线衍射，以及暴露于作为模型膜溶解蛋白的磷脂酶A2的作用。我们从磷脂酰乙醇胺与磷脂酰胆碱的等摩尔混合物开始研究，并用磷脂酰丝氨酸（PS）或磷脂酰肌醇（PI）掺杂该基质。结果表明，带有PS的膜在中尺度上更紧密地凝结，并在分子水平上周期性地组织。从这些模型开始，我们获得了两个模型真菌膜家族，它们被添加到这些磷脂基质麦角固醇中。结果表明，麦角固醇含量的水平对于模型膜结构及其耐久性至关重要。将麦角固醇的摩尔比从0更改为0。
更新日期：2019-11-19
中文翻译：
磷脂成分和麦角甾醇的存在在真菌膜适应恶劣环境条件中的作用-膜建模研究。
土壤真菌在分解死有机物和降解持久性有机污染物（POP）的环境中起着重要作用。土壤中疏水性POP的存在以及被竞争性微生物排泄到土壤溶液中的膜溶解性物质不断威胁着这些生物。为了在恶劣的环境中生存并抵消这些危害，真菌细胞必须严格控制其细胞膜中脂质的组成。然而，在真菌膜的情况下，其组成和物理性质之间的相关性还没有被完全理解。在我们的研究中，我们应用了由真菌膜和麦角固醇典型的磷脂形成的Langmuir单层膜作为通用模型膜。这些膜通过Langmuir技术进行了表征，布鲁斯特角显微镜和掠入射X射线衍射，以及暴露于作为模型膜溶解蛋白的磷脂酶A2的作用。我们从磷脂酰乙醇胺与磷脂酰胆碱的等摩尔混合物开始研究，并用磷脂酰丝氨酸（PS）或磷脂酰肌醇（PI）掺杂该基质。结果表明，带有PS的膜在中尺度上更紧密地凝结，并在分子水平上周期性地组织。从这些模型开始，我们获得了两个模型真菌膜家族，它们被添加到这些磷脂基质麦角固醇中。结果表明，麦角固醇含量的水平对于模型膜结构及其耐久性至关重要。将麦角固醇的摩尔比从0更改为0。
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