BACKGROUND While respiratory viral infections are recognized as a frequent cause of illness in hematopoietic stem cell transplantation (HSCT) recipients, HCoV-OC43 infections have rarely been investigated as healthcare-associated infections in this population. OBJECTIVES In this report, HCoV-OC43 isolates collected from HSCT patients were retrospectively characterized to identify potential clusters of infection that may stand for a hospital transmission. STUDY DESIGN Whole-genome and S gene sequences were obtained from nasal swabs using next-generation sequencing and phylogenetic trees were constructed. Similar identity matrix and determination of the most common ancestor were used to compare clusters of patient's sequences. Amino acids substitutions were analysed. RESULTS Genotypes B, E, F and G were identified. Two clusters of patients were defined from chronological data and phylogenetic trees. Analyses of amino acids substitutions of the S protein sequences identified substitutions specific for genotype F strains circulating among European people. CONCLUSIONS HCoV-OC43 may be implicated in healthcare-associated infections.

中文翻译：

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全基因组测序在造血干细胞移植单元中与护理相关的HCoV-OC43感染的分子研究中的用途。

背景技术尽管呼吸道病毒感染被认为是造血干细胞移植（HSCT）受者的常见疾病原因，但在该人群中，很少有人将HCoV-OC43感染作为医疗保健相关感染进行调查。目的在本报告中，对从HSCT患者收集的HCoV-OC43分离株进行了回顾性鉴定，以鉴定可能代表医院传播的潜在感染群。研究设计使用下一代测序技术从鼻拭子获得全基因组和S基因序列，并构建系统发育树。使用相似的身份矩阵和最常见祖先的确定来比较患者序列的簇。分析了氨基酸取代。结果鉴定出基因型B，E，F和G。从时间顺序数据和系统发育树中定义了两类患者。对S蛋白序列氨基酸取代的分析确定了欧洲人群中流行的基因型F株特有的取代。结论HCoV-OC43可能与医疗保健相关感染有关。