Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) have evolved into widely used and reliable cell sources for modeling cardiovascular channelopathies and for drug safety pharmacology. However, the electrophysiological and pharmacological applications of hiPSC-CMs are hampered by manual patch-clamp technique, which is labor-intensive and generates low output. The automated patch-clamp technique is showing potential to overcome this problem. Here, we describe a new dissociation method, with which we can harvest a vast number of single relaxed hiPSC-CMs with smooth membrane suited for automated patch-clamp. Using the automated whole-cell patch-clamp technology, we report a high success rate for cell capture and whole-cell access (around 70%). We are able to identify and record several currents and paced action potentials (APs) with different success rates, including Na⁺ current (I_Na), L-type Ca²⁺ current (I_CaL), two specific K⁺ currents, the transient outward K⁺ current (I_to) and the inward rectifier K⁺ current (I_K1). Moreover, we successfully applied dynamic current-clamp to virtually increase I_K1 for AP recordings. Our study suggests that automated patch-clamp technology could be used to investigate the relevant ionic currents and APs in hiPSC-CMs. The combination of automated patch-clamp and hiPSC-CM technologies promises a wide range of applications in the future.

人类诱导的多能干细胞衍生的心肌细胞（hiPSC-CM）已发展成为用于建模心血管疾病和药物安全药理学的广泛使用且可靠的细胞来源。但是，hiPSC-CM的电生理学和药理学应用受到手动贴片钳技术的阻碍，该技术费工费力且产量低。自动膜片钳技术显示了克服这一问题的潜力。在这里，我们描述了一种新的解离方法，借助该方法，我们可以收获大量具有平滑膜的单个松弛hiPSC-CM，适用于自动膜片钳。使用自动全细胞膜片钳技术，我们报告细胞捕获和全细胞访问的成功率很高（约70％）。⁺电流（I _Na），L型Ca ²⁺电流（I _CaL），两个特定的K ⁺电流，瞬态向外K ⁺电流（I _to）和向内整流器K ⁺电流（I _K1）。此外，我们成功地应用了动态电流钳来实际上增加AP记录的I _K1。我们的研究表明，自动膜片钳技术可用于研究hiPSC-CM中的相关离子电流和AP。自动化膜片钳和hiPSC-CM技术的结合，有望在未来广泛应用。