Revealing dynamics of gene expression variability in cell state space.,Nature Methods

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Revealing dynamics of gene expression variability in cell state space.
Nature Methods ( IF 36.1 ) Pub Date : 2019-11-18 , DOI: 10.1038/s41592-019-0632-3
Dominic Grün _{1,

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Affiliation

To decipher cell state transitions from single-cell transcriptomes it is crucial to quantify weak expression of lineage-determining factors, which requires computational methods that are sensitive to the variability of weakly expressed genes. Here, I introduce VarID, a computational method that identifies locally homogenous neighborhoods in cell state space, permitting the quantification of local variability in gene expression. VarID delineates neighborhoods with differential gene expression variability and reveals pseudo-temporal dynamics of variability during differentiation.

中文翻译：

揭示细胞状态空间中基因表达变异的动态。

为了破译单细胞转录组的细胞状态转变，量化谱系决定因子的弱表达至关重要，这需要对弱表达基因的可变性敏感的计算方法。在这里，我介绍了 VarID，这是一种计算方法，可识别细胞状态空间中的局部同质邻域，允许量化基因表达的局部变异性。VarID 描绘了具有差异基因表达变异性的邻域，并揭示了分化过程中变异性的伪时间动态。

更新日期：2019-11-18

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