Malaria remains one of the deadliest diseases worldwide and it is caused by the protozoan parasite Plasmodium spp . Parasite visualization is an important tool for the correct detection of malarial cases but also to understand its biology. Advances in visualization techniques promote new insights into the complex life cycle and biology of Plasmodium parasites. Live cell imaging by fluorescence microscopy or flow cytometry are the foundation of the visualization technique for malaria research. In this review, we present an overview of possibilities in live cell imaging of the malaria parasite. We discuss some of the state‐of‐the‐art techniques to visualize organelles and processes of the parasite and discuss limitation and advantages of each technique. © 2019 International Society for Advancement of Cytometry

中文翻译：

---

Live and Let Dye：可视化疟疾寄生虫恶性疟原虫的细胞室。

疟疾仍然是全世界最致命的疾病之一，它是由原生动物寄生虫疟原虫引起的。寄生虫可视化是正确检测疟疾病例的重要工具，也是了解其生物学的重要工具。可视化技术的进步促进了对疟原虫复杂生命周期和生物学的新见解寄生虫。通过荧光显微镜或流式细胞术进行活细胞成像是疟疾研究可视化技术的基础。在这篇综述中，我们概述了疟疾寄生虫活细胞成像的可能性。我们讨论了一些最先进的技术来可视化寄生虫的细胞器和过程，并讨论每种技术的局限性和优势。© 2019 国际细胞计量学促进会