Characterization and genomic analysis of emerging astroviruses causing fatal gout in goslings.,Transboundary and Emerging Diseases

当前位置： X-MOL 学术 › Transbound. Emerg. Dis. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Characterization and genomic analysis of emerging astroviruses causing fatal gout in goslings.
Transboundary and Emerging Diseases ( IF 3.5 ) Pub Date : 2019-11-17 , DOI: 10.1111/tbed.13410
Qinxi Chen ₁ , Xin Xu ₁ , Zhengli Yu ₁ , Chaoge Sui ₁ , Kejing Zuo ₂ , Guanglin Zhi ₂ , Jun Ji ₁ , Lunguang Yao ₁ , Yunchao Kan ₁ , Yingzuo Bi ₃ , Qingmei Xie ₃

Affiliation

Since February 2017, severe outbreaks of fatal gout caused by novel gosling astroviruses (GoAstVs) have occurred in several Chinese provinces, causing a considerable economic impact on the poultry industry. To assess the infection status of GoAstVs causing gout, 165 clinical samples were collected from goslings from seven farms located in different Chinese provinces, and they were screened for viral infection. Seven GoAstV strains were completely sequenced. The positive infection rates of GoAstV, goose parvovirus, reovirus, goose haemorrhagic polyomavirus and Tembusu virus were 100%, 9.69%, 3.64%, 0% and 0%, respectively, indicating the role of GoAstV in gout. The genomes of all seven GoAstV strains were 7170-nt long and encoded three open reading frames (ORFs), namely, ORF1a, ORF1b and ORF2. Sequence and phylogenetic analyses of the seven GoAstV strains showed that these were avastroviruses and were closely related to viruses classified within Avastrovirus 3 and turkey astrovirus 2. Moreover, the mutation rates of ORF1a and ORF2 were high, and ORF1a was highly mutated at amino acid loci 545-580. The tertiary structure of the mutated ORF2 protein was smooth, and its antigenic epitope was highly mutated, which may be related to the pathogenicity of the virus and caused by antibody pressure from the host. These findings enrich our understanding of the evolution of novel GoAstVs causing gout and their circulation as well as lay the foundation for the selection of vaccine strains.

更新日期：2019-11-17

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11